Zinnia sp pollen fluorescence

[Macro_Cosmos]

Maybe Zinnia elegans, not sure.



Acridine orange
GFP, CFP & TxRed
32x
Z6

[Adalbert]

very nice

[hkv]

Stunning!

[Scarodactyl]

You've been getting some awesome results with this!

[Lou Jost]

Really nice. Can you give equipment and setup details?

[JayMcClellan]

Congrats on the appreciation award, well deserved. It's interesting that there appear to be two different styles of these grains, some with what look like round depressions and some without. Any idea about the significance of that?

[Macro_Cosmos]

Really nice. Can you give equipment and setup details?

20x with 1.6x from a magnification changer, three channels as indicated by the description.
Inverted microscope, I modified my Wemacro to work with a prior Z-axis motor.
Outlined in 2 articles:
https://macrocosmosblog.wordpress.com/2 ... -part-1-2/
https://macrocosmosblog.wordpress.com/2 ... -part-2-2/
I had to modify the Wemacro controller because the current output was way too high.
Nothing too DIY/fancy/unusual.

Congrats on the appreciation award, well deserved. It's interesting that there appear to be two different styles of these grains, some with what look like round depressions and some without. Any idea about the significance of that?

I had the same question, my best guess is two slightly different species of pollen are represented, or they were at different maturity levels.
The pollen came from several flowers that "looked similar", rather than just one.