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Arcella (with gas?)

 
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Charles Krebs



Joined: 01 Aug 2006
Posts: 5846
Location: Issaquah, WA USA

PostPosted: Sat May 09, 2020 12:39 am    Post subject: Arcella (with gas?) Reply with quote

Well, it does appear so, but not because of a bad meal. Get to that later.

First... I found this Arcella racing around a recent wet mount. Now they don't move all that fast but I needed 33 images for the stack and during that time it would move from one side of the field of view to the other. (That may not look very far here, but it traveled about 1/2 its body width. These images are substantial crops). Not only that, it would also rotate at the same time. I thought I would give it a try anyway, and told myself if Zerene could align this mess I would have to give Rik an "atta-boy"! So kudos to Rik... his alignment routine did wonders

After taking the first image, I looked at other subjects on the slide and returned to the amoeba 5 minutes later. The test now had 4 large gas bubbles inside. So I quickly took the second image. Occasionally I have come across a testate amoeba with a bubble, but I was surprised to see this occur to this degree in one I had just been photographing.

Some hasty research provided some observations on why this occurs. Briefly... if the amoeba is unhappy with its surroundings, or its orientation, or the amount of oxygen in the water, the production of these bubbles inside the test allow it to float upward to a new location or orientation. It's interesting. Here are a couple of Google book excerpts that discuss it:

https://books.google.com/books?id=8QHQzFglZlkC&pg=PA193&lpg=PA193&dq=gas+bubbles+in+arcella&source=bl&ots=YXGrq2268D&sig=ACfU3U09nuUumzy-Qxz_fc8BngXdG4sq3w&hl=en&sa=X&ved=2ahUKEwjzluOxjaDpAhUDPH0KHZZvAvEQ6AEwCnoECAsQAQ#v=onepage&q=gas%20bubbles%20in%20arcella&f=false

https://books.google.com/books?id=6ODGvWAWtO8C&pg=PA216&lpg=PA216&dq=gas+bubbles+in+arcella&source=bl&ots=E4WVDCGnjO&sig=ACfU3U0K-a7nIGuHF-KnRRTVAM1yDaVnEQ&hl=en&sa=X&ved=2ahUKEwjzluOxjaDpAhUDPH0KHZZvAvEQ6AEwAHoECAgQAQ#v=onepage&q=gas%20bubbles%20in%20arcella&f=false

The images are DIC with 40/0.95 S Plan Apo. Canon EOS R camera.




Interesting how in the second image it appears that the large gas bubble may have displaced some of the amoeba, as you can see it around the periphery of the shell.
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Ken Ramos



Joined: 27 Jul 2006
Posts: 7172
Location: lat=35.4005&lon=-81.9841

PostPosted: Sat May 09, 2020 1:05 am    Post subject: Reply with quote

Your photographs are, as always, just mind boggling there Charles Cool and sitting here pondering this single celled animalcule within its test even more so. It is amazing how it can build so intricate a structure for itself to call home and to show such an intelligence as how to maintain it within its microhabitat.
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Bruce Taylor



Joined: 23 Jun 2011
Posts: 800
Location: Wakefield, Quebec / Ottawa, Ontario

PostPosted: Sat May 09, 2020 3:39 am    Post subject: Reply with quote

Amazing images, and an interesting post. I didn't know about the gas bubble trick. Very cool!
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Olympusman



Joined: 15 Jan 2012
Posts: 4476

PostPosted: Sat May 09, 2020 8:18 am    Post subject: Arcella Reply with quote

Nice shots. From what I have read, arcella can also make a bubble on one side to facilltate flipping over. Between making tests and this behavior I marvel at the capabilities for a creature with no brain.

Mike
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Sumguy01



Joined: 28 Jan 2013
Posts: 1498
Location: Ketchikan Alaska USA

PostPosted: Sat May 09, 2020 9:47 am    Post subject: Reply with quote

Applause Very nice.
Thanks for sharing.
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WalterD



Joined: 06 Jul 2015
Posts: 598
Location: Rotterdam, the Netherlands

PostPosted: Sun May 10, 2020 1:23 am    Post subject: Reply with quote

Beautiful images, interesting post. Smile
Thanks for sharing.
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micro_pix



Joined: 11 May 2012
Posts: 236

PostPosted: Sun May 10, 2020 1:21 pm    Post subject: Reply with quote

.

Great stuff! I was having a go at exactly the same thing the other day but eventually gave up. I ended up photographing an empty test. Thanks for showing that it can be done!

David
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Robert Berdan



Joined: 18 Oct 2017
Posts: 221
Location: Calgary

PostPosted: Mon May 11, 2020 6:33 pm    Post subject: Nice Focus stack Reply with quote

Nice Focus stack and lots of crisp detail. They move slowly, but I guess if you are making taking lots of images to stack and they rotate it can be challenging.
RB
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sebba28



Joined: 29 Dec 2011
Posts: 290
Location: Poland,Ireland

PostPosted: Mon May 11, 2020 6:39 pm    Post subject: Reply with quote

Amazing.
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pierre



Joined: 04 Jan 2010
Posts: 258
Location: France, Var, Toulon

PostPosted: Tue May 12, 2020 12:06 am    Post subject: Arcella (with gas?) Reply with quote

Indeed eyeopening Shocked

Excellent!

Thanks for sharing this great piece of work Charles.
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Pierre
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nanometer



Joined: 30 Apr 2016
Posts: 317
Location: Tucson, AZ

PostPosted: Tue May 12, 2020 10:51 pm    Post subject: Reply with quote

unbelievably excellent!
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Marek Mis



Joined: 10 Jul 2010
Posts: 2572
Location: Suwalki, Poland

PostPosted: Mon Jun 01, 2020 5:12 am    Post subject: Reply with quote

Very impressive images Charlie !
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