www.photomacrography.net :: View topic - Colpoda with confusing macronucleus?
www.photomacrography.net Forum Index
An online community dedicated to the practices of photomacrography, close-up and macro photography, and photomicrography.
Photomacrography Front Page Amateurmicrography Front Page
Old Forums/Galleries
 
 FAQFAQ   SearchSearch   MemberlistMemberlist   UsergroupsUsergroups   RegisterRegister 
 ProfileProfile   Log in to check your private messagesLog in to check your private messages   Log inLog in 
Colpoda with confusing macronucleus?

 
Post new topic   Reply to topic    www.photomacrography.net Forum Index -> Photography Through the Microscope
View previous topic :: View next topic  
Author Message
nanometer



Joined: 30 Apr 2016
Posts: 235
Location: Tucson, AZ

PostPosted: Sat Jun 08, 2019 6:55 am    Post subject: Colpoda with confusing macronucleus? Reply with quote

20x DIC, flash. Pretty compressed by the coverslip. It seems like a Colpoda, but I can't find a single image online showing this kind of macronucleus structure.

Back to top
View user's profile Send private message
Robert Berdan



Joined: 18 Oct 2017
Posts: 143
Location: Calgary

PostPosted: Sat Jun 08, 2019 9:19 am    Post subject: Nice photo Reply with quote

Hi beautiful photo with DIC - The Y shape nucleus is unusual. Also in this photo your background does not have the mottled appearance but is smooth. As you mentioned before some of us use Photoshop to clean the background and remove distracting elements, but most of the time the background I see with DIC is smooth as you show in this picture.

Very nice photo showing lots of detail. Did you buy the DIC for yourself or do you use a microscope in research? I thought I was one of the few to invest so much money in DIC optics. Now if I could only afford a confocal microscope Smile

Cheers
RB
Back to top
View user's profile Send private message Send e-mail Visit poster's website
nanometer



Joined: 30 Apr 2016
Posts: 235
Location: Tucson, AZ

PostPosted: Sat Jun 08, 2019 9:39 am    Post subject: Reply with quote

Hi Robert. Thanks for commenting. The mottled background was a consequence of my global use of the dehaze tool in Lightroom. So I don't do that anymore. Plus I'm not very good at cleaning my slides and coverslips, so the dehaze tool would emphasize that.

I'm just a hobbyist. Yes, this was a big expense, but that's how it goes with hobbies. No vacation this year (or last) and a DIC rig instead. I can't figure out why I have such wonder and amazement for each drop of water, but I do. This hobby combines my other passion which is photography, so it's a twofer.

I draw the line at confocal and epi-flour!
Back to top
View user's profile Send private message
Robert Berdan



Joined: 18 Oct 2017
Posts: 143
Location: Calgary

PostPosted: Sat Jun 08, 2019 11:45 am    Post subject: DIC Reply with quote

HI - thanks for your reply - I am the same way I love looking at micro-organisms from pond water - they are amazing and I am trying to promote microscopy more to others and show the beauty that exists in the micro-world.

I couldn't find a DIC microscope for under $10,000 - and decided that this might be my last chance in life, so I sold lots of my stuff and bought a Zeiss Axioscope with DIC and phase. I will be paying for it a for a few years, but I love it. I am trying to find a way to make some money back with it, but I will do it even if I don't. I have several refurbished microscopes now as well. Wide field flourescence isn't that expensive I purchased a used scope for $600 and I should take more pictures with it. Polarization is cheap to add and it results in some amazing colours and you already have this feature if you own a DIC scope - just pull out the DIC prisms and put a polarizer over the light source. I am going to rent some time on a confocal microscope because the pictures I have seen by Igor Siwanowicz - see his Stenors on this forum - they are so beautiful that I have to try it.

I look forward to seeing more of your pictures. What I enjoy about this forum is that lots of folks are sharing their passions, information and amazing pictures and I have made a few friends that share this passion.

Best wishes
RB
Back to top
View user's profile Send private message Send e-mail Visit poster's website
actinophrys



Joined: 24 Apr 2014
Posts: 51

PostPosted: Sat Jun 08, 2019 12:06 pm    Post subject: Reply with quote

This doesn't seem to have the groove typical of colpodids, either. You can partly see the mouth and oral cilia in the upper left of the photo. I think their shape mark this as a hymenostome, maybe something like Ophryoglena, which often have elliptical macronuclei but in some species more elongate ones.
Back to top
View user's profile Send private message Visit poster's website
nanometer



Joined: 30 Apr 2016
Posts: 235
Location: Tucson, AZ

PostPosted: Sat Jun 08, 2019 12:20 pm    Post subject: Reply with quote

Thanks Actin. The mouth didn't look right to me, but I'm not very familiar with IDs. I'll check into your suggestion.

Steve
Back to top
View user's profile Send private message
nanometer



Joined: 30 Apr 2016
Posts: 235
Location: Tucson, AZ

PostPosted: Sat Jun 08, 2019 12:47 pm    Post subject: Reply with quote

Yes--your suggestion looks like a better fit. I see mention of a light sensor near the mouth opening. Perhaps I'm stretching here, but I recall seeing an odd-looking copperish bridge on one of the 20x images. Here is a crop. I wonder if this is the sensor?

Back to top
View user's profile Send private message
Display posts from previous:   
Post new topic   Reply to topic    www.photomacrography.net Forum Index -> Photography Through the Microscope All times are GMT - 7 Hours
Page 1 of 1

 
Jump to:  
You cannot post new topics in this forum
You cannot reply to topics in this forum
You cannot edit your posts in this forum
You cannot delete your posts in this forum
You cannot vote in polls in this forum


Powered by phpBB © 2001, 2005 phpBB Group