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Freshwater snail radula

 
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blepharopsis



Joined: 28 Nov 2014
Posts: 143
Location: Virginia, USA

PostPosted: Wed May 15, 2019 10:12 am    Post subject: Freshwater snail radula Reply with quote

Hi there, been a while. Here is some snail tongue imaged with a LSCM, 40x NA 1.3. The width of the image is 303 um. Depth color-coded. Enjoy!



...

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Jacek



Joined: 02 Oct 2011
Posts: 5302
Location: Poland

PostPosted: Wed May 15, 2019 11:21 am    Post subject: Reply with quote

Wow SUPER ! Shocked
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https://photos.app.goo.gl/MV9IynUtBdaKkVj53
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blepharopsis



Joined: 28 Nov 2014
Posts: 143
Location: Virginia, USA

PostPosted: Wed May 15, 2019 11:51 am    Post subject: Reply with quote

Thanks, Jacek. Dang, I forgot how to post stuff to this very fine forum, I'm turning into my grandpa...
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carlos.uruguay



Joined: 23 Feb 2012
Posts: 5215
Location: Uruguay - Montevideo - America del Sur

PostPosted: Wed May 15, 2019 4:45 pm    Post subject: Reply with quote

Super nice!!!
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Sumguy01



Joined: 28 Jan 2013
Posts: 1345
Location: Ketchikan Alaska USA

PostPosted: Wed May 15, 2019 9:00 pm    Post subject: Reply with quote

Applause Very nice.
Love the colors.
Thanks for sharing.
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WalterD



Joined: 06 Jul 2015
Posts: 515
Location: Rotterdam, the Netherlands

PostPosted: Wed May 15, 2019 11:31 pm    Post subject: Reply with quote

Spectacular!!! Shocked
I'm very glad to see your new pictures posted, the series you made a couple of years ago are still my all time favourites.
What confocal laser scanning microscope are you using?

Best regards
Walter
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grgh



Joined: 09 Mar 2013
Posts: 223
Location: Lancashire. UK

PostPosted: Thu May 16, 2019 3:59 am    Post subject: as subject Reply with quote

Very nice photos,
how on earth do you get pictures of a snails tongue?

something different,..... I enlarged your photo and then, by accident moved the screen up and down really fast. what an effect? nearly mind blowing.
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used to do astronomy.
and photography.
Zeiss Universal Phase contrast.
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Smokedaddy



Joined: 07 Oct 2006
Posts: 1379
Location: Phoenix, Arizona

PostPosted: Thu May 16, 2019 8:54 am    Post subject: Reply with quote

Excellent!
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zed



Joined: 07 Feb 2019
Posts: 17
Location: Houston, TX

PostPosted: Thu May 16, 2019 9:01 am    Post subject: Reply with quote

Very neat! Is this auto-fluorescence or some IHC labeling?
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blepharopsis



Joined: 28 Nov 2014
Posts: 143
Location: Virginia, USA

PostPosted: Thu May 16, 2019 9:06 am    Post subject: Reply with quote

So, guys, snail tongues are absolutely fascinating and a great subject for polarized light or fluorescent/confocal photomicrography - and each one is different! Google "radula" and check out all the images. Getting a tongue out of a poor mollusk is a bit messy - you have to liquefy the body using either household products like bleach or Drano (sodium or potassium hydroxide) or trypsinate it into oblivion (my preferred method). The radula (and sometimes upper beak-like "jaw" are the only hard parts within the mollusk's head.
Can anyone hook me up with some marine snails like periwinkles or small whelks? I'm being totally serious Smile
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micro_pix



Joined: 11 May 2012
Posts: 187

PostPosted: Fri May 17, 2019 2:33 am    Post subject: Reply with quote

Beautifully done! I’m looking forward to seeing a periwinkle tongue now.

Dave
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blepharopsis



Joined: 28 Nov 2014
Posts: 143
Location: Virginia, USA

PostPosted: Fri May 17, 2019 5:49 am    Post subject: Reply with quote

Zed - Thanks Smile I used Congo Red, beats autofluorescence almost every time in terms of signal strength.
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pwnell



Joined: 18 Dec 2009
Posts: 2000
Location: Tsawwassen, Canada

PostPosted: Mon May 27, 2019 12:53 pm    Post subject: Reply with quote

Breathtakingly beautiful as always.
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Robert Berdan



Joined: 18 Oct 2017
Posts: 143
Location: Calgary

PostPosted: Tue Jun 04, 2019 9:59 am    Post subject: Depth colour coding Reply with quote

HI - beautiful images, could you please explain or provide a link to depth colour coding. Are you using Image J to apply colour to a black and white image?

Thank you
RB
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blepharopsis



Joined: 28 Nov 2014
Posts: 143
Location: Virginia, USA

PostPosted: Thu Jun 06, 2019 12:37 pm    Post subject: Reply with quote

Hi Robert,
yes, I used Fiji/ImageJ Hyperstacks function (I think it's under "Image") - it offers a whole bunch of color options/gradients, and you will double that by inverting the stacks.
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