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Jacek



Joined: 02 Oct 2011
Posts: 5303
Location: Poland

PostPosted: Wed Jan 09, 2019 12:47 pm    Post subject: Various Reply with quote














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Harald



Joined: 13 May 2011
Posts: 551
Location: Steinberg, Norway

PostPosted: Wed Jan 09, 2019 1:12 pm    Post subject: Reply with quote

Hi Jacek,
Great set of images as always.
Love the 3rd one of the moquito, amazing details and light.

Thanks for sharing Very Happy
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Harald

Lier Fotoklubb / NSFF
AFIAP / CPS
BGF / GMV
http://www.500px.com/blender11
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MASZEK



Joined: 04 Jul 2018
Posts: 65
Location: POLAND

PostPosted: Wed Jan 09, 2019 4:03 pm    Post subject: Reply with quote

Hi Jacek.
My whole comment: great photos.
All. Very Happy
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anne



Joined: 05 Dec 2014
Posts: 130

PostPosted: Thu Jan 10, 2019 12:17 am    Post subject: Reply with quote

Absolutely stunning images!
Thank you for sharing.
anne
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santiago



Joined: 25 Sep 2018
Posts: 108
Location: Nijmegen, The Netherlands

PostPosted: Thu Jan 10, 2019 5:22 am    Post subject: Reply with quote

Amazing images!
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Lou Jost



Joined: 04 Sep 2015
Posts: 3596
Location: Ecuador

PostPosted: Thu Jan 10, 2019 5:28 am    Post subject: Reply with quote

These are such beautiful photos. I only wish that you would always include some minimal information about the techniques and objectives/lenses for each of these inspiring images.
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Lou Jost
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viktor j nilsson



Joined: 01 Mar 2013
Posts: 114
Location: Lund, Sweden

PostPosted: Thu Jan 10, 2019 8:08 am    Post subject: Reply with quote

Just... wow. I agree with Lou, I'd really appreciate at least a brief note about the objectives and illumination for these images. In the meantime, I drool.
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Jacek



Joined: 02 Oct 2011
Posts: 5303
Location: Poland

PostPosted: Thu Jan 10, 2019 10:59 am    Post subject: Reply with quote

Thank you very much Smile

EPI (two Ikea lights + diffuser)

1. EPI + Pol (dia) (Zeiss Apo 6.3/20)
2. Pol (Zeiss Apo 6.3/20)
3. EPI (Nikon CFN PlanApo 10/0.45)
4. Pol (Nikon CFN PlanApo 10/0.45)
5. autoFL (UV) (Nikon CFN PlanApo 40/0.95)
6. autoFL (UV) (Zeiss Apo 6.3/20)
7. EPI (Nikon CFN Plan 4/0.13)

Next set:

1. BF (Nikon CFN PlanApo 10/0.45)
2. BF (Zeiss Apo 6.3/20)
3. autoFL (UV) (Zeiss Apo 6.3/20)
4. EPI (Nikon CFN PlanApo 10/0.45)
5. EPI (Zeiss Apo 6.3/20)
6. DIC (Nikon CFN Plan Fluor 40/0.75)
7. DIC (Nikon CFN Plan Fluor 40/0.75)














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Lou Jost



Joined: 04 Sep 2015
Posts: 3596
Location: Ecuador

PostPosted: Thu Jan 10, 2019 11:17 am    Post subject: Reply with quote

Thanks very much for the added information and for the second set! It is much appreciated.
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Lou Jost
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ChrisR
Site Admin


Joined: 14 Mar 2009
Posts: 8323
Location: Near London, UK

PostPosted: Thu Jan 10, 2019 11:35 am    Post subject: Reply with quote

Super pictures, again Smile

I believe you are using afocal method, yes?
When you change from Zeiss to Nikon, do you change eyepieces?

I apologize if you have already covered this!
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Jacek



Joined: 02 Oct 2011
Posts: 5303
Location: Poland

PostPosted: Thu Jan 10, 2019 12:30 pm    Post subject: Reply with quote

Yes, I'm still doing it afokal, I do not change my glasses.
I have Chinese glasses 10X/20 with a rejected pupil.
I use Nikon CFWN 10X/20 glasses only for DIC.












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pbraub



Joined: 02 Feb 2018
Posts: 54

PostPosted: Thu Jan 10, 2019 12:40 pm    Post subject: Reply with quote

Spectacular images, thank you Jacek!

What lightsource do you use for UV excitation? Do you excite through the objective or from the side?

Kind regards
Peter
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Robert Berdan



Joined: 18 Oct 2017
Posts: 143
Location: Calgary

PostPosted: Thu Jan 10, 2019 9:55 pm    Post subject: Amazing Photomicrographs Reply with quote

Exceptional images Jacek you are an inspiration. Well done.
Rob
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Sumguy01



Joined: 28 Jan 2013
Posts: 1345
Location: Ketchikan Alaska USA

PostPosted: Thu Jan 10, 2019 11:49 pm    Post subject: Reply with quote

Applause Excellent photos.
Thanks for sharing.
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Jacek



Joined: 02 Oct 2011
Posts: 5303
Location: Poland

PostPosted: Fri Jan 11, 2019 12:43 am    Post subject: Reply with quote

Thanks Smile

I use fluorescence on two microscopes. On one FL epi (LED lighting 3W 365 nm (UV) and 460 nm (blue)).
On the second one I have FL dia, one diode 365 nm on it, a converted condenser, enlarging the diode + UG5 filter and barrier filter.
In addition, for small magnifications (10X lens and small) I use two illuminators that I have made (365 LEDs + UG5 excitation filters) - this solution is very efficient lighting.






















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