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Lilly pollen
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JH



Joined: 09 Mar 2013
Posts: 1216
Location: Vallentuna, Stockholm, Sweden

PostPosted: Sun Oct 07, 2018 10:49 am    Post subject: Lilly pollen Reply with quote

Lilly pollen, Nikon BD Plan Apo 150x NA 0.90. For this picture I used darkfield light. The high NA makes every dot, spot, line... on the sensor visible so I did some retouching both before and after stacking. I darkened the background and sharpened the picture. Canon 5D II, Zerene Stacker, Adobe LR and PS.

Best regards
Jörgen Hellberg




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rjlittlefield
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Joined: 01 Aug 2006
Posts: 19887
Location: Richland, Washington State, USA

PostPosted: Sun Oct 07, 2018 11:31 am    Post subject: Reply with quote

Spectacular!

So, this was illuminated with light coming up through the specimen?

--Rik
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JH



Joined: 09 Mar 2013
Posts: 1216
Location: Vallentuna, Stockholm, Sweden

PostPosted: Sun Oct 07, 2018 11:50 am    Post subject: Reply with quote

rjlittlefield wrote:
Spectacular!

So, this was illuminated with light coming up through the specimen?

--Rik


Thanks!

It is a Bright field/Darkfield (BD) EPI-light lens so light from above.

Best regards
Jörgen Hellberg
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Saul



Joined: 31 Jan 2011
Posts: 1427
Location: Naperville, IL USA

PostPosted: Sun Oct 07, 2018 12:07 pm    Post subject: Reply with quote

Jörgen , incredible photos, especially stereo.
What setup you are using ?
Thanks,
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rjlittlefield
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Joined: 01 Aug 2006
Posts: 19887
Location: Richland, Washington State, USA

PostPosted: Sun Oct 07, 2018 12:21 pm    Post subject: Reply with quote

JH wrote:
It is a Bright field/Darkfield (BD) EPI-light lens so light from above.

Ah, epi darkfield. Thanks!

--Rik
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Lou Jost



Joined: 04 Sep 2015
Posts: 3614
Location: Ecuador

PostPosted: Sun Oct 07, 2018 9:36 pm    Post subject: Reply with quote

Really beautiful! These kinds of pictures would be great tools for studying pollen samples when the species is unknown, especially ancient pollen.
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Lou Jost
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JH



Joined: 09 Mar 2013
Posts: 1216
Location: Vallentuna, Stockholm, Sweden

PostPosted: Mon Oct 08, 2018 8:16 am    Post subject: Reply with quote

Saul wrote:

What setup you are using ?

Hi Saul, I used a Nikon Optiphot microscope. The microscope have a 2.5x projection lens, so a lot of empty magnification and 375x on sensor. The 150x lens only have 0.3 mm working distance.

In this case I wanted to show the colours of the pollen grain. For ”clean” black and white pictures I think that a GIF (green) filter is a good option.

Best regards
Jörgen Hellberg
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Jacek



Joined: 02 Oct 2011
Posts: 5325
Location: Poland

PostPosted: Mon Oct 08, 2018 10:04 am    Post subject: Reply with quote

Nice Very Happy
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JH



Joined: 09 Mar 2013
Posts: 1216
Location: Vallentuna, Stockholm, Sweden

PostPosted: Mon Oct 08, 2018 3:00 pm    Post subject: Reply with quote

Thanks for the comments!

This is with the 40x BD Plan apo lens, cropped.

Best regards
Jörgen Hellberg





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Olympusman



Joined: 15 Jan 2012
Posts: 4165

PostPosted: Tue Oct 09, 2018 10:42 am    Post subject: Lily Pollen Reply with quote

That is crazy beautiful. Really loved the 3D.

Mike
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WalterD



Joined: 06 Jul 2015
Posts: 518
Location: Rotterdam, the Netherlands

PostPosted: Fri Oct 12, 2018 1:57 pm    Post subject: Reply with quote

Hi Jorgen,

Very interesting and aesthetic. The 40x red ones are my favourite!
Thanks for sharing.
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JH



Joined: 09 Mar 2013
Posts: 1216
Location: Vallentuna, Stockholm, Sweden

PostPosted: Fri Oct 12, 2018 2:13 pm    Post subject: Reply with quote

Thanks Mike and WalterD!

Here is a link to a Video https://youtu.be/s9Eh4rDLE_8

I used the new Zerne Stacker function Stere/Rocking "Use conical path"

Best regards
Jörgen Hellberg
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Marek Mis



Joined: 10 Jul 2010
Posts: 2554
Location: Suwalki, Poland

PostPosted: Mon Oct 15, 2018 9:48 am    Post subject: Reply with quote

Nice images Jorgen ! The red ones remind me slightly odd strawberries Smile

Marek
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JH



Joined: 09 Mar 2013
Posts: 1216
Location: Vallentuna, Stockholm, Sweden

PostPosted: Sat Oct 20, 2018 2:58 am    Post subject: Reply with quote

Thanks Marek

Here is a panorama made from four stacked pictures. I used the BD plan apo 150x lens.

Best regards
Jörgen Hellberg


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Beatsy



Joined: 05 Jul 2013
Posts: 1522
Location: Malvern, UK

PostPosted: Sat Oct 20, 2018 12:54 pm    Post subject: Reply with quote

Spectacular! Practically a separate genre - photomiacrography, or miacro for short Smile
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