Lilly pollen

Images made through a microscope. All subject types.

Moderators: Chris S., Pau, rjlittlefield, ChrisR

JH
Posts: 1277
Joined: Sat Mar 09, 2013 9:46 am
Location: Vallentuna, Stockholm, Sweden
Contact:

Lilly pollen

Post by JH »

Lilly pollen, Nikon BD Plan Apo 150x NA 0.90. For this picture I used darkfield light. The high NA makes every dot, spot, line... on the sensor visible so I did some retouching both before and after stacking. I darkened the background and sharpened the picture. Canon 5D II, Zerene Stacker, Adobe LR and PS.

Best regards
Jörgen Hellberg


Image
Image
Jörgen Hellberg, my webbsite www.hellberg.photo

rjlittlefield
Site Admin
Posts: 20752
Joined: Tue Aug 01, 2006 8:34 am
Location: Richland, Washington State, USA
Contact:

Post by rjlittlefield »

Spectacular!

So, this was illuminated with light coming up through the specimen?

--Rik

JH
Posts: 1277
Joined: Sat Mar 09, 2013 9:46 am
Location: Vallentuna, Stockholm, Sweden
Contact:

Post by JH »

rjlittlefield wrote:Spectacular!

So, this was illuminated with light coming up through the specimen?

--Rik
Thanks!

It is a Bright field/Darkfield (BD) EPI-light lens so light from above.

Best regards
Jörgen Hellberg
Jörgen Hellberg, my webbsite www.hellberg.photo

Saul
Posts: 1615
Joined: Mon Jan 31, 2011 11:59 am
Location: Naperville, IL USA
Contact:

Post by Saul »

Jörgen , incredible photos, especially stereo.
What setup you are using ?
Thanks,

rjlittlefield
Site Admin
Posts: 20752
Joined: Tue Aug 01, 2006 8:34 am
Location: Richland, Washington State, USA
Contact:

Post by rjlittlefield »

JH wrote:It is a Bright field/Darkfield (BD) EPI-light lens so light from above.
Ah, epi darkfield. Thanks!

--Rik

Lou Jost
Posts: 4340
Joined: Fri Sep 04, 2015 7:03 am
Location: Ecuador
Contact:

Post by Lou Jost »

Really beautiful! These kinds of pictures would be great tools for studying pollen samples when the species is unknown, especially ancient pollen.

JH
Posts: 1277
Joined: Sat Mar 09, 2013 9:46 am
Location: Vallentuna, Stockholm, Sweden
Contact:

Post by JH »

Saul wrote: What setup you are using ?
Hi Saul, I used a Nikon Optiphot microscope. The microscope have a 2.5x projection lens, so a lot of empty magnification and 375x on sensor. The 150x lens only have 0.3 mm working distance.

In this case I wanted to show the colours of the pollen grain. For ”clean” black and white pictures I think that a GIF (green) filter is a good option.

Best regards
Jörgen Hellberg
Jörgen Hellberg, my webbsite www.hellberg.photo

Jacek
Posts: 5353
Joined: Sun Oct 02, 2011 7:00 am
Location: Poland

Post by Jacek »

Nice :D

JH
Posts: 1277
Joined: Sat Mar 09, 2013 9:46 am
Location: Vallentuna, Stockholm, Sweden
Contact:

Post by JH »

Thanks for the comments!

This is with the 40x BD Plan apo lens, cropped.

Best regards
Jörgen Hellberg


Image

Image
Jörgen Hellberg, my webbsite www.hellberg.photo

Olympusman
Posts: 4507
Joined: Sun Jan 15, 2012 12:31 pm

Lily Pollen

Post by Olympusman »

That is crazy beautiful. Really loved the 3D.

Mike
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

WalterD
Posts: 608
Joined: Mon Jul 06, 2015 12:01 pm
Location: Rotterdam, the Netherlands
Contact:

Post by WalterD »

Hi Jorgen,

Very interesting and aesthetic. The 40x red ones are my favourite!
Thanks for sharing.

JH
Posts: 1277
Joined: Sat Mar 09, 2013 9:46 am
Location: Vallentuna, Stockholm, Sweden
Contact:

Post by JH »

Thanks Mike and WalterD!

Here is a link to a Video https://youtu.be/s9Eh4rDLE_8

I used the new Zerne Stacker function Stere/Rocking "Use conical path"

Best regards
Jörgen Hellberg
Jörgen Hellberg, my webbsite www.hellberg.photo

Marek Mis
Posts: 2584
Joined: Sat Jul 10, 2010 9:56 am
Location: Suwalki, Poland
Contact:

Post by Marek Mis »

Nice images Jorgen ! The red ones remind me slightly odd strawberries :)

Marek

JH
Posts: 1277
Joined: Sat Mar 09, 2013 9:46 am
Location: Vallentuna, Stockholm, Sweden
Contact:

Post by JH »

Thanks Marek

Here is a panorama made from four stacked pictures. I used the BD plan apo 150x lens.

Best regards
Jörgen Hellberg

Image
Jörgen Hellberg, my webbsite www.hellberg.photo

Beatsy
Posts: 1619
Joined: Fri Jul 05, 2013 3:10 am
Location: Malvern, UK

Post by Beatsy »

Spectacular! Practically a separate genre - photomiacrography, or miacro for short :)

Post Reply