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Testate amoebae?

 
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enricosavazzi



Joined: 21 Nov 2009
Posts: 1078
Location: Stockholm, Sweden

PostPosted: Mon Jul 02, 2018 11:19 am    Post subject: Testate amoebae? Reply with quote

Still waiting for 100x oil objective, so this is with UPlanApo 40x on Olympus BX50, strongly cropped. The first is a Zerene stack of 6 manually shot images. I originally included both sides of the test in the stack, but the resulting stack was confusing. This is only one side of the test plus the outer wall seen on-end. DIC, with the kind of colored haloes that suggest to me the test is mineralized.



The second is much larger and less cropped, a single image showing the test surface pattern.



PS - Both subjects come from a semi-dry moss and soil sample placed in a container with water and left exposed to indirect daylight for a couple of weeks.
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enricosavazzi



Joined: 21 Nov 2009
Posts: 1078
Location: Stockholm, Sweden

PostPosted: Tue Jul 03, 2018 2:42 am    Post subject: Reply with quote

Arcella, from the same sample. Lightly fixed with diluted Lugol's.


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enricosavazzi



Joined: 21 Nov 2009
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Location: Stockholm, Sweden

PostPosted: Tue Jul 03, 2018 9:46 am    Post subject: Reply with quote

One more. This one is so small that the image below is a 1:1 pixel crop from a 16 Mpixel original.


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houstontx



Joined: 31 Jul 2015
Posts: 226

PostPosted: Tue Jul 03, 2018 10:08 am    Post subject: Reply with quote

Very cool, looks like one of the ones found here:
http://penard.de/Explorer/Cercozoa/Imbricatea/
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enricosavazzi



Joined: 21 Nov 2009
Posts: 1078
Location: Stockholm, Sweden

PostPosted: Wed Jul 04, 2018 1:27 am    Post subject: Reply with quote

houstontx wrote:
Very cool, looks like one of the ones found here:
http://penard.de/Explorer/Cercozoa/Imbricatea/

Good to see some (very clean) SEM pictures of similar tests. They are good examples of how translucent objects render in completely different ways in SEM and optical microscope. And of course DIC adds its own "interpretation" of 3D structure.

I wonder how they would look dry in incident illumination at 100x or so (without DIC) with a Mitutoyo M Plan Apo 100x or similar objective. Unfortunately at present I can only get up to 20x with these objectives.
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Pau
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Joined: 20 Jan 2010
Posts: 4500
Location: Valencia, Spain

PostPosted: Wed Jul 04, 2018 3:55 am    Post subject: Reply with quote

Very good for a 100% crop at 40X!
Your DIC is running well Very Happy
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carlos.uruguay



Joined: 23 Feb 2012
Posts: 5013
Location: Uruguay - Montevideo - America del Sur

PostPosted: Wed Jul 11, 2018 6:08 am    Post subject: Reply with quote

Super!!!!
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