Lacrymaria?

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GaryB
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Lacrymaria?

Post by GaryB »

After cultivating my 'pond' aka tupperware tub for a few weeks, these creatures have appeared. I think it may be a Lacrymaria but I'm not certain.
Nikon 20x via samsung galaxy s6

Video here:
https://www.youtube.com/watch?v=zFCY6yqiFek
Screen grab from above video
Image

Bruce Taylor
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Location: Wakefield, Quebec / Ottawa, Ontario
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Post by Bruce Taylor »

Nice! :) It's a dileptid (note that the "mouth" is down at the base of the proboscis, and the body features a long row of contractile vacuoles). Macronuclear nodules are inconspicuous, so it's probably Dileptus (I think that central body is too small to be the macronucleus of Monomacrocaryon).
It Came from the Pond (Blog): http://www.itcamefromthepond.com/

eward1897
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Location: Minnesota, US

haptorid ciliate

Post by eward1897 »

Nice work with a cell phone and your microscope. I see Bruce Taylor says probable Dileptus sp. and he is probably right.

Somewhat confusingly, Haptoria are a mostly carnivorous subclass within the class Litostomatea of ciliate protozoa, but Haptoria are also an order within the subclass, so haptorid and litostomate can be synonyms, or not. See http://rspb.royalsocietypublishing.org/ ... /1738/2625 for a recent genomic analysis of the relationships among the members of the group.

I've often known I was looking at some haptorid, but could not be sure about the genus. They are all long necked predators and prowl about looking for prey. Several times I wondered if I'd seen Lacrymaria, but once I finally found Lacrymaria olor the extremely long narrow neck and "bristly" mouth at the end were unmistakable, and I had not seen it previously.

Richard Howey has a nice article about Lacymaria (and pretty much everything else under the sun) at Micscape, microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.org.uk/mag/art ... hlac2.html. There is a nice family portrait and much other good information about haptorid ciliates in LACRYMARIA OLOR AND THE LITOSTOMATA – BEAUTIFUL CILIATES WITH NASTY DISPOSITIONS at theislandpond.com

A good on-line source for identifying common ciliates is Ciliated protozoa : an illustrated guide to the species used as biological indicators in freshwater biology / Harmut Bick at http://apps.who.int/iris/handle/10665/40066

http://www.photomacrography.net/forum/u ... aweb_2.jpg
old AO phase optics on Reichert microstar iv ('crappiest microscope ever produced by the hand of man' )

GaryB
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Joined: Fri Jul 29, 2016 12:18 pm

Post by GaryB »

Great info guys.

Here's one that is different, it has the long neck but a stumpy body. Not sure if it's a variant, juvenile or what. Also, I should have my lighting sorted out later today to fix the ugly LED flickering.
https://www.youtube.com/watch?v=_qzzdjKAduc

Bruce Taylor
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Re: haptorid ciliate

Post by Bruce Taylor »

eward1897 wrote:Somewhat confusingly, Haptoria are a mostly carnivorous subclass within the class Litostomatea of ciliate protozoa, but Haptoria are also an order within the subclass, so haptorid and litostomate can be synonyms, or not.
Yup, relationships within Litostomatea are confusing, and many important groups are still unresolved (Peter Vd'acny titled his 2014 paper on phylogeny of haptorians "The Chaos Prevails" :D ). Until the dust settles, the best practice is to follow the consensus taxonomy in Gao et al., 2016: https://www.nature.com/articles/srep24874 The authors (which include Q. Zhang, lead author on the multigene analysis you cited above) accept three subclasses under Litostomatea: Haptoria, Trichostomatia and Rhynchostomatia.

Dileptids are in the last group, the subclass Rhynchostomatia (a robustly supported monophyletic group).

Lacrymariids are in the subclass Haptoria, a rather messy and almost certainly paraphyletic group (see Huang et al., 2017).

A pretty picture, from Gao et al., 2016:

Image
Last edited by Bruce Taylor on Sat Apr 14, 2018 4:59 pm, edited 1 time in total.
It Came from the Pond (Blog): http://www.itcamefromthepond.com/

Bruce Taylor
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Joined: Thu Jun 23, 2011 4:49 pm
Location: Wakefield, Quebec / Ottawa, Ontario
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Post by Bruce Taylor »

GaryB wrote:Great info guys.

Here's one that is different, it has the long neck but a stumpy body. Not sure if it's a variant, juvenile or what. Also, I should have my lighting sorted out later today to fix the ugly LED flickering.
https://www.youtube.com/watch?v=_qzzdjKAduc
Most likely, the tail is rounded because this is the forward half (proter) of a recent division. Length of the proboscis can vary quite a bit from one specimen to the next. The proboscis will be very short on the rear half (opisthe) of a recent division. Also, dileptids often lose and regrow their "trunks," and overall body shape can change quite dramatically under coverslip pressure.
It Came from the Pond (Blog): http://www.itcamefromthepond.com/

GaryB
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Joined: Fri Jul 29, 2016 12:18 pm

Post by GaryB »

Thanks, Bruce.

I don't know how people can even begin to fathom the complexities of these family trees, and this is just one of hundreds, if not thousands. It's fun though because as my little pond has evolved I'm seeing more species arrive and a few that seem to have vanished as it transitioned from a putrid, smelly biohazard into the clean and flourishing thing it is now. Over half I hadn't seen before.

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