Zeiss DIC Setup

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neurodoc
Posts: 57
Joined: Wed Aug 29, 2012 8:59 pm

Zeiss DIC Setup

Post by neurodoc »

I want to convert one of my scopes for DIC. I have a WL and couple of Standards set up for PC. Since the WL seems the most versatile, I was thinking of using that one for DIC. It also has an optovar with pol analyzer, a substage polarizer, and a circular rotating stage. I also have the Zeiss 47-30-59-9902 intermediate tube, which I'd substitute for the optovar. I know this will accept the DIC III Slider, which I understand will be difficult to find.

I prefer to use this system rather than the more recent one that requires getting several nosepiece objective sliders that, although perhaps functionally better would wind up quite expensive.

My questions are:

1) which DIC turret condensers would I need to get?

2) Which objectives would I be able to use in combination with the DIC III slider and that condenser?

Thanks for your help!
Man sieht nur, was man weiss--Goethe

Ichthyophthirius
Posts: 1152
Joined: Thu Mar 07, 2013 5:24 am

Post by Ichthyophthirius »

Hi,

The components you require are listed here http://www.mikroskopie-forum.de/index.php?topic=2567.0 (in German) or here http://www.the-ultraphot-shop.org.uk/faq.htm (in English).

0) The good news is, you can also use the slider II (which is easier to find). It merely requires you to rotate your intermediate tube horizontally by 180 degrees.

1) There is only one option; the INKO condenser with NA 1.4 achr.-apl. pol front lens. It comes in several versions, most look like this one http://www.microscopeitaly.it/wp-conten ... i/inko.jpg They never have a part number engraved.

2) Most condensers have prisms I, II and III; designed for objectives Plan 10, Plan 40 and Plan 100 (only certain serial numbers will work!) as well as Neofluar 10 and Neofluar 40. Some other objectives will also work, e.g. the Planapo 40/1,0 Oil. In rare cases the condenser also has prism IIII for the Plan 6,3 which has to be used with front lens apl. pol 0,63 in which case the other prims won't work normally (i.e. you have the change the front lens).

Where are you based? In the US it is often cheapest to buy a complete Phomi with DIC and transfer the parts to your WL :wink:

Regards, Ichty

neurodoc
Posts: 57
Joined: Wed Aug 29, 2012 8:59 pm

Post by neurodoc »

Thank you, Ichty! You answered all my questions, and the links were also incredibly helpful. I've got all the qppropriate objectives, including the Plan and Neofluars. I also have an APO 40 1.0 Oel (with the 0.6-1.0 compensating ring) and since it isn't designated as PlanAPO I'm not sure it will work, but I'll try it when I get the condenser and slider. It seems odd that they'd make a non-Plan APO, but I guess there might be a call for that. I'm glad I can use the II slider, since I have seen those around. And thanks for the Phomi tip; I'll check that out.

I'm in the USA, west coast (Bay Area).

Thanks again!
Man sieht nur, was man weiss--Goethe

Ichthyophthirius
Posts: 1152
Joined: Thu Mar 07, 2013 5:24 am

Post by Ichthyophthirius »

neurodoc wrote:It seems odd that they'd make a non-Plan APO, but I guess there might be a call for that.
Hi,

The Apo 40/1,0 is simply an older product. The Planapo 40/1,0 was a leap forward (1960s?).

The Apo is supposed to be better for darkfield (fewer lenses) but I don't know how it performs for DIC.

Regards, Ichty

Eddie
Posts: 141
Joined: Thu Oct 18, 2012 5:00 am

Post by Eddie »

Check this post out. It uses unconventional objectives which will give you DIC but this is using different Zeiss DIC slides but I'm sure it would also give you choices with just the single beam DIC slide III or II
http://www.photomacrography.net/forum/v ... hp?t=31435

neurodoc
Posts: 57
Joined: Wed Aug 29, 2012 8:59 pm

Post by neurodoc »

Thanks for the reference, Eddie. It will be very useful once I get the basic setup and am looking to expand beyond the limited range of Zeiss "pre-approved" objectives.
Man sieht nur, was man weiss--Goethe

Pau
Site Admin
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Location: Valencia, Spain

Post by Pau »

I use the first Zeiss DIC type, but mostly in an unconventional way:
http://www.photomacrography.net/forum/v ... hp?p=99260
The Planapo 63/1.4 and 25/0.65 also work pretty well with this system.
Pau

neurodoc
Posts: 57
Joined: Wed Aug 29, 2012 8:59 pm

Post by neurodoc »

Thanks, Pau. Great information from all of you, and I hope to put it all to good use.

BTW, on another topic...been going through all my Zeiss odds and ends and have quite a few funky KPL's with delaminated doublet groups. As these are already shot but their glass is otherwise fine, I've been meaning to try my hand at de-cementing them and re-cementing them with a good quality Canada balsam. Anyone tried that here? The alignment shouldn't be a problem because most of them are uniformly cylindrical with elements of the same diameter in each group.
Man sieht nur, was man weiss--Goethe

Pau
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Post by Pau »

Ichthyophthirius wrote:The good news is, you can also use the slider II (which is easier to find). It merely requires you to rotate your intermediate tube horizontally by 180 degrees
I assume this is based in actual experience. It would had be nice to know few years ago!.
Actually with my Standard I do need to put the Inko III slider in NE--> SW position inversely to the SW-->NE pictured in the Zeiss info and Eddie's setup pictures. Is possible that my condenser came from an inverted microscope?
Pau

Ichthyophthirius
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Joined: Thu Mar 07, 2013 5:24 am

Post by Ichthyophthirius »

Hi Pau,

It is mentioned in the German link.

I also have a slider II and the DIC works fine. It's widely accepted that the slider II is fully functional. If you look at Eddie's picture http://www.photomacrography.net/forum/u ... tems_1.jpg you see the arrows on the sliders pointing in opposite directions. If you turn slider II by 180 degrees vs slider III the arrow matches the arrow on the condenser.

However, I don't have a slider III and haven't done a side-by-side comparison myself.

Regards, Ichty

Ichthyophthirius
Posts: 1152
Joined: Thu Mar 07, 2013 5:24 am

Post by Ichthyophthirius »

Pau wrote:Actually with my Standard I do need to put the Inko III slider in NE--> SW position inversely to the SW-->NE pictured in the Zeiss info and Eddie's setup pictures. Is possible that my condenser came from an inverted microscope?
The arrows on condenser and slider have to point in the same direction. So for slider III, NE--> SW should be correct.

Pau
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Post by Pau »

Eddie shows it mounted at SW-->NE position on his black WL
http://www.photomacrography.net/forum/v ... 113#182113
And also I recall seeing it at the Zeiss inko pages I did scan (I haven't them at hand, I'm not at home), but I use it with the arrows pointing to the same direction as you say
Pau

Ichthyophthirius
Posts: 1152
Joined: Thu Mar 07, 2013 5:24 am

Post by Ichthyophthirius »

Hi Pau,

I assume that Eddie is using a slider II in that image.

For slider III, it looks like this, the other way around: http://fs5.directupload.net/images/170111/6qms473d.jpg

Pau
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Joined: Wed Jan 20, 2010 8:57 am
Location: Valencia, Spain

Post by Pau »

Yes, this is how I use it. Thanks!
Pau

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