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Phlox

 
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JH



Joined: 09 Mar 2013
Posts: 1216
Location: Vallentuna, Stockholm, Sweden

PostPosted: Wed May 03, 2017 1:07 pm    Post subject: Phlox Reply with quote

Phlox

Mitutoyo M plan 5x and 20x, Canon 6D, Zerene stacker.

Best regards Jörgen Hellberg






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zzffnn



Joined: 22 May 2014
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PostPosted: Wed May 03, 2017 1:22 pm    Post subject: Reply with quote

Beautifully done! Thank you.
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Jacek



Joined: 02 Oct 2011
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PostPosted: Wed May 03, 2017 11:09 pm    Post subject: Reply with quote

Very nice, first super !
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Johann



Joined: 02 Aug 2016
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Location: South Africa

PostPosted: Wed May 03, 2017 11:50 pm    Post subject: Reply with quote

Amazing - super job Smile
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carlos.uruguay



Joined: 23 Feb 2012
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PostPosted: Thu May 04, 2017 1:12 pm    Post subject: Reply with quote

Very nice!
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JH



Joined: 09 Mar 2013
Posts: 1216
Location: Vallentuna, Stockholm, Sweden

PostPosted: Sat May 06, 2017 5:32 am    Post subject: Reply with quote

Thanks for the nice comments! Very Happy
The last picture is a crop from the stereo so the 20x delivers a lot of detail.
Best regards Jörgen Helllberg
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hkv



Joined: 02 Jan 2013
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Location: Sweden

PostPosted: Sat May 06, 2017 8:46 am    Post subject: Reply with quote

Super images! Colors are fantastic! Thanks for sharing!
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JH



Joined: 09 Mar 2013
Posts: 1216
Location: Vallentuna, Stockholm, Sweden

PostPosted: Sun May 07, 2017 10:08 am    Post subject: Reply with quote

Thanks hkv

Pollen are quite difficult. Nikon Mplan ELWD 100x, 250x on sensor.

Best regards
Jörgen


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JH



Joined: 09 Mar 2013
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Location: Vallentuna, Stockholm, Sweden

PostPosted: Sun May 07, 2017 11:13 am    Post subject: Reply with quote

I do like to take photos of plants. At high magnification, some of the most interesting details are the pollen grains. They are quite easy to observe but annoyingly difficult to stack. I normally do fresh unmounted pollen grains and uses diverse types of light diffused LED, EPI, DF. After buying those UV Convoy LEDs (Thanks Charles!) I had one more option. It turned out that in some situations I can get quit a decent pollen picture with those UV-LEDs and a Baader Fringe Killer filter.

Best regards Jörgen Hellberg


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zzffnn



Joined: 22 May 2014
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Location: Texas USA

PostPosted: Sun May 07, 2017 12:26 pm    Post subject: Reply with quote

Jörgen,

What makes pollen grains difficult to stack - perhaps out-of-focus halos (contamination from layers above and below the in-focus layer)?

And how do you overcome those difficulties - do you do layers of Pmax stacks (photos 1-10 as stack layer #1, photos 11-20 as stack layer #2, ect) then combine all layers in a final stack?

Thank you for sharing!
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JH



Joined: 09 Mar 2013
Posts: 1216
Location: Vallentuna, Stockholm, Sweden

PostPosted: Sun May 07, 2017 12:39 pm    Post subject: Reply with quote

zzffnn wrote:
Jörgen,

What makes pollen grains difficult to stack - perhaps out-of-focus halos (contamination from layers above and below the in-focus layer)?

And how do you overcome those difficulties - do you do layers of Pmax stacks (photos 1-10 as stack layer #1, photos 11-20 as stack layer #2, ect) then combine all layers in a final stack?

Thank you for sharing!


Yes usually a lot of work with halos and transparent surfaces. A lot of pictures goes directly to the dustbin. But the auto fluorescence pollen grains stacked nicely.

Regards Jörgen
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Charles Krebs



Joined: 01 Aug 2006
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Location: Issaquah, WA USA

PostPosted: Mon May 08, 2017 10:42 pm    Post subject: Reply with quote

Excellent set of images! Thanks for posting these.
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WalterD



Joined: 06 Jul 2015
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Location: Rotterdam, the Netherlands

PostPosted: Tue May 09, 2017 11:34 am    Post subject: Reply with quote

Excellent and inspiring; number one is my favourite.
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carlos.uruguay



Joined: 23 Feb 2012
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Location: Uruguay - Montevideo - America del Sur

PostPosted: Thu May 11, 2017 5:34 am    Post subject: Reply with quote

Excellent!
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