solartje wrote:ok now i understand the lengths..
my goal is checking my blood in live blood smears for educational purposes. me and my wife have positive serology for several bacteria. i've been studying them for 2 years now and now i want to see them with my own eyes (even if it means small odds without dna staining or cultivation). hence the darkfield and the 400X and 1000x need.
i'm gonna start with darkfield live view and safe staining techniques in brightfields.
But honestly... now i found this forum and i look at all the pictures ,maybe photomicrography could become a new hobby. its fascinating. i'm constantly looking for things to put under my microscope and amazed at the new worlds i'm seeing :-) diatoms especially are amazing and i love the compositions of them. Does this mean i'm getting hooked?
ps with the NA of 1.00 i could get max of 1000x without losing resolution right? or is that only in theory. thats why i was thinking of the combo 60/0.90 and X16 instead of the 100/oil/iris and the X8. both will give almost same resolution and almost 1000X total mag. but it would save me the trouble of having to oil the objectives.
PS2, my next problem will be the light. it has the standard 6v 15w halogen lamp and i guess it wont be strong enough for 100x darkfield?
i'm reading alot about that, and not sure whats the best option. diy led replacement , find a used 100W 12V old zeiss parts but that will be costly, or find a 30W 6V but guess thats not enough either. (http://zeiss-campus.magnet.fsu.edu/arti ... logen.html ) guess i need a led with 4k lumens wich is ALOT. ) pau did you use led lightning and if not is the 15w lamp really a big problem for darkfield or only like 10% less or something in contrast?)
Edit: my slides arrived and did a test on 40x. set the köler correct so all planes are equal. in BF the 40X works nice. So i decided to make a darkfield stop and test the 40x/65 in df with the 0,9 cond it came with. It worked , because without specimen it was black and with i saw something, but the light intensity is WAY to low. even if i turn my 15w to max, i barely can see something in 40x, so i can only imagine with the 100x obj this will be even worse. sight. the big Q, how many lumens is needed :-) anyone with a 100x obj in ultradf that can tell me how many lumens there setup has?