Aquatic crustaceans - cuticle + muscles

Images made through a microscope. All subject types.

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blepharopsis
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Aquatic crustaceans - cuticle + muscles

Post by blepharopsis »

Fluorescent phalloidins (actin markers) have huge penetration issues; the organisms have to be partially "opened" and incubation times of days are usually necessary.
In the second sample I was trying to visualize the nervous system (Acetylated tubulin is a great axonal marker, usually). Instead I labeled muscle attachment sites. D'oh...

Image
Marine amphipod - Calc. White (cuticle), Texas Red-phalloidin (muscles). 10x NA 0.45

Image
Fresh-water copepod - Calc. White (cuticle), Alexa-488-phalloidin (muscles), acetylated tubulin (immuno-staining, red). 10x NA 0.45

gpmatthews
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Post by gpmatthews »

=D> absolutely superb! The copepod is especially wow!!
Graham

Though we lean upon the same balustrade, the colours of the mountain are different.

Jacek
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Post by Jacek »

I collect jaw off the floor :shock:

arturoag75
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Post by arturoag75 »

Impressive again :shock:

carlos.uruguay
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Post by carlos.uruguay »

Perfect

Litonotus
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Post by Litonotus »

the last one - no words... just one: how...?
my FB page

I'm looking for the the extemely rare V-IM magnification changer for the E800 scope. If you have seen a listing or have one for sale please let me know.

Harald
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Post by Harald »

Hi there blepharopsis,
This is great images! Love them!
Kind Regards
Harald

Lier Fotoklubb / NSFF
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pwnell
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Post by pwnell »

Fantastic as always.

RogelioMoreno
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Post by RogelioMoreno »

Beautiful, specially the second one.

Rogelio

Olympusman
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Crustaceans

Post by Olympusman »

Beauty! The first is an amphipod.

Mike
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

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