www.photomacrography.net :: View topic - Aquatic crustaceans - cuticle + muscles
www.photomacrography.net Forum Index
An online community dedicated to the practices of photomacrography, close-up and macro photography, and photomicrography.
Photomacrography Front Page Amateurmicrography Front Page
Old Forums/Galleries
 
 FAQFAQ   SearchSearch   MemberlistMemberlist   UsergroupsUsergroups   RegisterRegister 
 ProfileProfile   Log in to check your private messagesLog in to check your private messages   Log inLog in 
Aquatic crustaceans - cuticle + muscles

 
Post new topic   Reply to topic    www.photomacrography.net Forum Index -> Photography Through the Microscope
View previous topic :: View next topic  
Author Message
blepharopsis



Joined: 28 Nov 2014
Posts: 143
Location: Virginia, USA

PostPosted: Fri Dec 26, 2014 2:23 pm    Post subject: Aquatic crustaceans - cuticle + muscles Reply with quote

Fluorescent phalloidins (actin markers) have huge penetration issues; the organisms have to be partially "opened" and incubation times of days are usually necessary.
In the second sample I was trying to visualize the nervous system (Acetylated tubulin is a great axonal marker, usually). Instead I labeled muscle attachment sites. D'oh...


Marine amphipod - Calc. White (cuticle), Texas Red-phalloidin (muscles). 10x NA 0.45


Fresh-water copepod - Calc. White (cuticle), Alexa-488-phalloidin (muscles), acetylated tubulin (immuno-staining, red). 10x NA 0.45
Back to top
View user's profile Send private message Visit poster's website
gpmatthews



Joined: 03 Aug 2006
Posts: 1040
Location: Horsham, W. Sussex, UK

PostPosted: Fri Dec 26, 2014 2:40 pm    Post subject: Reply with quote

Applause absolutely superb! The copepod is especially wow!!
_________________
Graham

Though we lean upon the same balustrade, the colours of the mountain are different.
Back to top
View user's profile Send private message Visit poster's website
Jacek



Joined: 02 Oct 2011
Posts: 5348
Location: Poland

PostPosted: Fri Dec 26, 2014 2:55 pm    Post subject: Reply with quote

I collect jaw off the floor Shocked
_________________
https://photos.app.goo.gl/MV9IynUtBdaKkVj53
Back to top
View user's profile Send private message Send e-mail
arturoag75



Joined: 05 Feb 2010
Posts: 1597
Location: italy

PostPosted: Sat Dec 27, 2014 3:20 am    Post subject: Reply with quote

Impressive again Shocked
_________________
www.microscopeitaly.it
Back to top
View user's profile Send private message Visit poster's website
carlos.uruguay



Joined: 23 Feb 2012
Posts: 5258
Location: Uruguay - Montevideo - America del Sur

PostPosted: Sat Dec 27, 2014 5:27 am    Post subject: Reply with quote

Perfect
Back to top
View user's profile Send private message Send e-mail Visit poster's website
Litonotus



Joined: 20 Jun 2011
Posts: 1394
Location: Poland

PostPosted: Sat Dec 27, 2014 5:43 am    Post subject: Reply with quote

the last one - no words... just one: how...?
_________________
my FB page

I'm looking for the the extemely rare V-IM magnification changer for the E800 scope. If you have seen a listing or have one for sale please let me know.
Back to top
View user's profile Send private message Visit poster's website
Harald



Joined: 13 May 2011
Posts: 576
Location: Steinberg, Norway

PostPosted: Sat Dec 27, 2014 11:02 am    Post subject: Reply with quote

Hi there blepharopsis,
This is great images! Love them!
_________________
Kind Regards
Harald

Lier Fotoklubb / NSFF
AFIAP / CPS
BGF / GMV
http://www.500px.com/blender11
Back to top
View user's profile Send private message Visit poster's website
pwnell



Joined: 18 Dec 2009
Posts: 2000
Location: Tsawwassen, Canada

PostPosted: Sat Dec 27, 2014 11:46 am    Post subject: Reply with quote

Fantastic as always.
Back to top
View user's profile Send private message
RogelioMoreno



Joined: 20 Nov 2009
Posts: 2962
Location: Panama

PostPosted: Sat Dec 27, 2014 1:52 pm    Post subject: Reply with quote

Beautiful, specially the second one.

Rogelio
Back to top
View user's profile Send private message Send e-mail
Olympusman



Joined: 15 Jan 2012
Posts: 4257

PostPosted: Sat Dec 27, 2014 2:21 pm    Post subject: Crustaceans Reply with quote

Beauty! The first is an amphipod.

Mike
_________________
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA
Back to top
View user's profile Send private message
Display posts from previous:   
Post new topic   Reply to topic    www.photomacrography.net Forum Index -> Photography Through the Microscope All times are GMT - 7 Hours
Page 1 of 1

 
Jump to:  
You cannot post new topics in this forum
You cannot reply to topics in this forum
You cannot edit your posts in this forum
You cannot delete your posts in this forum
You cannot vote in polls in this forum


Powered by phpBB © 2001, 2005 phpBB Group