Aquatic crustaceans - cuticle + muscles

Images made through a microscope. All subject types.

Moderators: Chris S., Pau, rjlittlefield, ChrisR

Post Reply
blepharopsis
Posts: 148
Joined: Fri Nov 28, 2014 12:39 pm
Location: Virginia, USA
Contact:

Aquatic crustaceans - cuticle + muscles

Post by blepharopsis »

Fluorescent phalloidins (actin markers) have huge penetration issues; the organisms have to be partially "opened" and incubation times of days are usually necessary.
In the second sample I was trying to visualize the nervous system (Acetylated tubulin is a great axonal marker, usually). Instead I labeled muscle attachment sites. D'oh...

Image
Marine amphipod - Calc. White (cuticle), Texas Red-phalloidin (muscles). 10x NA 0.45

Image
Fresh-water copepod - Calc. White (cuticle), Alexa-488-phalloidin (muscles), acetylated tubulin (immuno-staining, red). 10x NA 0.45

gpmatthews
Posts: 1040
Joined: Thu Aug 03, 2006 10:54 am
Location: Horsham, W. Sussex, UK
Contact:

Post by gpmatthews »

=D> absolutely superb! The copepod is especially wow!!
Graham

Though we lean upon the same balustrade, the colours of the mountain are different.

Jacek
Posts: 5353
Joined: Sun Oct 02, 2011 7:00 am
Location: Poland

Post by Jacek »

I collect jaw off the floor :shock:

arturoag75
Posts: 1597
Joined: Fri Feb 05, 2010 1:05 am
Location: italy
Contact:

Post by arturoag75 »

Impressive again :shock:

carlos.uruguay
Posts: 5350
Joined: Thu Feb 23, 2012 8:05 pm
Location: Uruguay - Montevideo - America del Sur
Contact:

Post by carlos.uruguay »

Perfect

Litonotus
Posts: 1394
Joined: Mon Jun 20, 2011 1:48 am
Location: Poland
Contact:

Post by Litonotus »

the last one - no words... just one: how...?
my FB page

I'm looking for the the extemely rare V-IM magnification changer for the E800 scope. If you have seen a listing or have one for sale please let me know.

Harald
Posts: 601
Joined: Fri May 13, 2011 10:33 am
Location: Steinberg, Norway
Contact:

Post by Harald »

Hi there blepharopsis,
This is great images! Love them!
Kind Regards
Harald

Lier Fotoklubb / NSFF
AFIAP / CPS
BGF / GMV
http://www.500px.com/blender11

pwnell
Posts: 2026
Joined: Fri Dec 18, 2009 4:59 pm
Location: Tsawwassen, Canada

Post by pwnell »

Fantastic as always.

RogelioMoreno
Posts: 2962
Joined: Fri Nov 20, 2009 11:24 am
Location: Panama

Post by RogelioMoreno »

Beautiful, specially the second one.

Rogelio

Olympusman
Posts: 4503
Joined: Sun Jan 15, 2012 12:31 pm

Crustaceans

Post by Olympusman »

Beauty! The first is an amphipod.

Mike
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

Post Reply