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I am not able to get a very sharp image even with post adjustments.
can any one give me some advice? thanks in advance. Sam
Moderators: rjlittlefield, ChrisR, Chris S., Pau
the condenser was set to 0.7-0.75
No not that one. For a microscope use m/2NA. You have an intermediate image (the actual image formed by the objective) of 50X, and the NA used is a little less than the full stated aperture on the objective (let's say about 0.825). So your effective aperture is roughly efff/30. But the primary point is not to come up with a hard "value" but to simply realize that you are unavoidably into "diffraction territory", so images taken at significantly larger effective apertures (if used for comparison) will almost certainly appear "sharper".does this formula work:
Effective Aperture = Lens Aperture x (1 + Magnification)
any adjustment I can do to reduce or prevent the diffraction for the high NA Objectives?
All the more reason to do a comparison with shutter speeds of 2-3 seconds or longer. This would at least determine whether vibration is a problem.the cover glass might not be the problem because I am seeing a crisp image via the eyepieces.