Photomac,
Thanks for answering my lengthy list of questions. For me, your answers ruled out certain potential issues, and highlighted the possibility of others. Rik has already responded on the meaty issues, but I'll venture a few other thoughts.
While taking more shots than necessary isn’t a problem per se, it can add complexity—especially when you add slabbing to the equation. If one is having issues, eliminating unneeded complexity is a good idea while solving those issues. I’m a proponent of slabbing to deal with certain difficult subjects, but would not expect to find it useful in this particular photographic situation. And it seems to me that you have an order of magnitude more shots than you need, here. As Rik said, at f/8, given your subject field size, you’d have about 45mm of DOF—nearly two inches. So if you use a one-inch stacking increment, you should have lots of overlap. How deep is this spray of flowers, front to back—maybe four to six inches? If so, I’d be thinking along the lines of starting with maybe six images, with an inch or so of focus separation between each. Once you get that image with no issues, feel free to try finer increments and see if you find improvement.
I haven’t used a Nikon 60mm micro, but have lots of experience with Nikon 105mm and 200mm micro lenses. These have a nice, long focus throw, and for a short stack with one-inch increments, I’d probably turn the focus knob by hand. I do it often enough in the field, and find it pretty easy; I don’t overworry about having identical increments—just look through the viewfinder and guesstimate by eye.
Your stacked image strikes me as suffering from veiling flare, and thinking about your lighting arrangement, I wonder if the sheet behind the flowers is bouncing too much light onto the front of your lens? A litmus test might be to make a quick stack of the flower spray with no background or background light, possibly even adding a black velvet or similar background, if you have it. If the subject is sharper without the background and background light, you have flare. In that case, I’d try angling the white background to bounce much of the light away from the lens, and see if that clears up the flare.
And thank you for the suggestion on the slab software - do you know if any of them work with a Mac? I was under the impression that the SlabberJockey did not, but I could be wrong.
Sorry, I should have noticed in your original post—and from your forum name—that you work on a Mac. SlabberJockey is something I knocked together for my own use. Once it was in existence, I figured that I might as well offer it to others who might find it useful. But I use Windows PCs, and know very little about MacIntosh computers. SlabberJockey is a Microsoft Access application, and can be run under the free runtime version of Access. To the best of my knowledge, Access is available only for PCs—though perhaps some Mac wizard could teach us otherwise. However, that's probably unnecessary, as Bill Eldridge wrote a very nice program called
Bugslabber, which runs on both Macs and Windows computers. The need for a Mac-compatible program was one of the main reasons Bill wrote it. I briefly tried Bugslabber in a beta version, and thought it seemed pretty good. Last I heard, you just need to PM Bill if you want to try his program. As I recall, if you like Bugslabber and intend to keep using it, he asks that you make, on the honor system, a $10 donation to a charitable organization that fosters girls’ science education.
But again I’ll say that for this particular image, I don’t see a clear benefit to be had by slabbing.
PMax slabs first and then try doing a DMap process on the those sub stacks.
I do this frequenty, in order to get the advantages of each approach (PMax and DMap), while avoiding their disadvantages. For a 100x, 1500-image stack of a difficult subject that Zerene Stacker seems to miraculously assemble out of a cloud of blur—yes, try this, especially for a low-key image where the darker sections have increased potential to accumulate noise. But for a subject like yours—high key, capable of being shot with a shallow stack—no. Zerene Stacker can probably handle this situation in its sleep.
I was avoiding doing the PMax slabs because I had read that PMax introduces color variations as well as noise and contrast issues.
While technically true, I think too much worry exists about this PMax trait—which I hesitate to call an “issue.” I use PMax and DMap about equally, and for situations where PMax works better, correcting color and contrast takes me less than a minute in Photoshop, with a curves layer. Your D800 sensor should produce very little noise, at base ISO, so even if it accumulates, I think you’ll usually find it not to be a problem. And if it is, you can always perform noise reduction on the input images or the stacked image.
Best,
--Chris