With prey (single shot):

Waiting for prey (37 frames focus stacked):

A closer crop of the above:

All images are with Canon T1i camera and Canon EF 100mm F/2.8 L IS USM lens and Teleplus Pro 300 1.4X teleconverter, f/8, 1/16 second).
The focus stack was automatically acquired using the Magic Lantern firmware add-on, 3 min 4 sec for 37 frames (5 seconds/frame), no tethering required. StepSize 2 with1 step per frame. The spider did move during the stack, but only while it was out of focus and I was getting the rest of the foreground where the spider wasn't. This made it easy to get a clean composite by Stack Selected and retouching in Zerene. The composite is mostly PMax, some DMap in areas where the brights were haloing.
Here's the setup. The tripod is a Manfrotto Pro 055XPROB with a Manfrotto 410 geared head. It's great for this sort of work because the geared head lets me dial in exactly the framing I want, even though in this case the camera is standing back well over a foot from the subject.

--Rik