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Olympusman



Joined: 15 Jan 2012
Posts: 3672

PostPosted: Tue Jan 17, 2012 9:45 am    Post subject: Greetings Reply with quote

After lurking your website for the past couple of years, I felt it was time to join the party. Greetings all. I must say I have been very impressed by the expertise, information exchange, images and cordiality shown on this website and I'll do my best to contribute what I can.
I'm Mike and live in Bethlehem, Pennsylvania. I have been an amateur microscopist for a bit over nine years. I contribute to Micscape once or twice a year. If you want to see some of my articles, Google "Michael Reese Much." I work as a product support specialist in digital cameras and digital audio for Olympus Imaging America. We have some images by Charlie Krebs and Wim van Egmond from the Bioscapes competitions in one of our hallways here at corporate headquarters.
My interests in the micro/macro world are focus stacked insects, cross-polarized chemical crystals, and plant and animal tissues. I have an AO Spencer microtome, and one of these days I'll get the blades sharpened and learn how to parafin mount speciments, so someday I may be posting some tissue sections. For now I use purchased "teaching " slides for my biological images.
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Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA
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naturephoto1



Joined: 13 Nov 2011
Posts: 509
Location: Breinigsville, PA

PostPosted: Tue Jan 17, 2012 9:48 am    Post subject: Re: Greetings Reply with quote

Olympusman wrote:
After lurking your website for the past couple of years, I felt it was time to join the party. Greetings all. I must say I have been very impressed by the expertise, information exchange, images and cordiality shown on this website and I'll do my best to contribute what I can.
I'm Mike and live in Bethlehem, Pennsylvania. I have been an amateur microscopist for a bit over nine years. I contribute to Micscape once or twice a year. If you want to see some of my articles, Google "Michael Reese Much." I work as a product support specialist in digital cameras and digital audio for Olympus Imaging America. We have some images by Charlie Krebs and Wim van Egmond from the Bioscapes competitions in one of our hallways here at corporate headquarters.
My interests in the micro/macro world are focus stacked insects, cross-polarized chemical crystals, and plant and animal tissues. I have an AO Spencer microtome, and one of these days I'll get the blades sharpened and learn how to parafin mount speciments, so someday I may be posting some tissue sections. For now I use purchased "teaching " slides for my biological images.


Welcome to the forum Mike. I do not live too far away from you; I am in Breinigsville, PA on the other side of Allentown.

Rich
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Richard A. Nelridge

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Olympusman



Joined: 15 Jan 2012
Posts: 3672

PostPosted: Wed Jan 18, 2012 9:50 am    Post subject: Reply with quote

Howdy, Rich. Nice to be on board. Maybe we can meet sometime. I live on the mountain just above the Olympus campus.
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Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA
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abpho



Joined: 17 Aug 2011
Posts: 1426
Location: Earth

PostPosted: Wed Jan 18, 2012 4:20 pm    Post subject: Reply with quote

Welcome.
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Contest_Guru



Joined: 10 May 2013
Posts: 5
Location: Canada

PostPosted: Sun Jun 23, 2013 11:45 pm    Post subject: Re: Greetings Reply with quote

Welcome to Photomacrography forum Olympusman
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Olympusman



Joined: 15 Jan 2012
Posts: 3672

PostPosted: Mon Jun 24, 2013 5:49 am    Post subject: Greetings Reply with quote

Thanks, Contest_Guru. Since Olympus Imaging America fired myself and 32 other other their top people (including all of the camera repair people) in March as a cost-cutting measure, I've had a lot of free time so I have been participating more on this site.
Olympus cameras are now being repaired in Ciudad Juarez, Mexico by a contractor.
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Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA
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discomorphella



Joined: 01 Oct 2006
Posts: 600
Location: NW USA

PostPosted: Sun Jul 07, 2013 9:03 am    Post subject: Reply with quote

Hi Mike,

Welcome. I have been making histological preparations for decades as an amateur (although I started out as a pathology tech, so I did have a professional introduction). Although I do use paraffin as an embedding medium, and various methacrylate formulations, I would reccommend that you try using polyester wax. It is more expensive, but it also doesn't require any solvent beyond isopropanol or ethanol, so your entire embedding protocol is much simpler (no clearing solvents required). Good luck, hope to see some more histo pictures on the forum.

Regards,

Dave (discomorphella)
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Olympusman



Joined: 15 Jan 2012
Posts: 3672

PostPosted: Mon Jul 08, 2013 9:47 am    Post subject: Microtome Reply with quote

Thanks, Dave. I'm going to try a combination of paraffin and beeswax for starters. I've done initial blade sharpening using a very fine stone and then burnishing with diamond paste on a plate of glass. Then I'm going to try my final buff with diamond paste on Dremel polishing pads which are kind of like felt.
A few years ago, a gou started a three part series on prepping specimens for microtome sectioning but he never posted Part Three, which was the stuff about mounting the sections to a slide and dissolving the paraffin. From everything I've reasd, the paraffin is dissolved out of the section using Xylol, which I have.
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Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA
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Olympusman



Joined: 15 Jan 2012
Posts: 3672

PostPosted: Sun Nov 03, 2013 8:46 am    Post subject: A small horn toot Reply with quote

I've been informed by the RMS that one of my images has been selected for the 2014 Royal Microscopical Society Calendar -- Male Imperial Moth Antenna detail



This is the third year in a row one of my images has been featured on the RMS calendar.
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Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA
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ChrisR
Site Admin


Joined: 14 Mar 2009
Posts: 7719
Location: Near London, UK

PostPosted: Sun Nov 03, 2013 11:20 am    Post subject: Reply with quote

Well done Michael, that one's a beaut!
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