Now I'm happy

Starting out in microscopy? Post images and ask questions relating to the microscope and get answers from our more advanced users on the subject.

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Simon W
Posts: 153
Joined: Fri Jul 01, 2011 4:15 am
Location: Melbourne, Australia
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Now I'm happy

Post by Simon W »

A variety of pics from my first 'serious' photo session with the new setup. No stacking here, just more practice and fiddling with the oblique patch.

Small clover, 2x obj
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I never realised the complexity of a clover:
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First remotely decent 40x pic (well, more like 500x or so into the sensor, yes I'll need to use Charles' tips on measuring..)
Image

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Liked the colours in this one:

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Apple, 40x obj, you can see the fibres of the flesh. Gawd, I feel so ignorant compared to you biologists!

Image
Simon W
EOS 5D Mk 3; Olympus BH-2; Zerene Stacker
Melbourne, Australia

bromodomain
Posts: 156
Joined: Sun Jan 02, 2011 11:50 am

Post by bromodomain »

2nd one is my favourite.

Good job man. I'd like to see more. Hey try some pond water and see whats lurking in there.

Simon W
Posts: 153
Joined: Fri Jul 01, 2011 4:15 am
Location: Melbourne, Australia
Contact:

Post by Simon W »

Thanks. I don't know what NOT to try, it's so satisfying. I gotta drive a couple of Kays and get some ocean samples. Or just swab my sneakers..
Simon W
EOS 5D Mk 3; Olympus BH-2; Zerene Stacker
Melbourne, Australia

Charles Krebs
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Joined: Tue Aug 01, 2006 8:02 pm
Location: Issaquah, WA USA
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Post by Charles Krebs »

Simon,

I strongly encourage everyone to play around with oblique illumination. The specimens you show here are very thick, and have great contrast by themselves. You will see a difference from "straight" brightfield, but it may not be that significant. The real value from oblique comes more when you work with subjects that are thinner, relatively "clear", and have a refractive index closer to the medium where they exist. You will see this if/when you get into the tiny water critters.

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