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DIC + epifluorescence

 
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bromodomain



Joined: 02 Jan 2011
Posts: 155

PostPosted: Thu Jul 14, 2011 11:03 am    Post subject: DIC + epifluorescence Reply with quote

This is one of my first attempts at DIC ever and since I was missing parts I had to improvise so I used a circular polarizer with a λ/4 compensator in conjunction with fluorescence. Thats a rat nerve that I cut and I think this is the zone of myelin transition between the central and peripheral nervous systems.

Unfortunately the camera wasn't working properly and I was acquiring images with a different exposure than the preview one Sad


DNA stained with DAPI

I used an optigrid panel to spatially attenuate the UV beam and thus managed to illuminate only half the field. You can see the birefringent Schwann cells and the difference in DNA (or cellular density) across the transition.

I wish I had the 20x/0.75 Planapo nomarski prism because this is my favourite objective Cool This image was acquired with a 10x/0.3 Plan-NEOFLUAR.


Last edited by bromodomain on Thu Aug 11, 2011 5:55 am; edited 2 times in total
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Cactusdave



Joined: 09 Jun 2009
Posts: 1631
Location: Bromley, Kent, UK

PostPosted: Fri Jul 15, 2011 6:00 am    Post subject: Reply with quote

Looks like you may have some interesting optical effects going on there. I'd like to see some more pictures using the circular polariser and quarter wave compensator.
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bromodomain



Joined: 02 Jan 2011
Posts: 155

PostPosted: Fri Jul 15, 2011 3:02 pm    Post subject: Reply with quote

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Last edited by bromodomain on Thu Aug 11, 2011 5:54 am; edited 1 time in total
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bromodomain



Joined: 02 Jan 2011
Posts: 155

PostPosted: Sat Jul 16, 2011 3:59 pm    Post subject: Reply with quote

Cactusdave wrote:
Looks like you may have some interesting optical effects going on there.

If I'm using circularly polarised light wouldn't that be C-DIC?
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