

Shot as 6 frames handheld stack, Canon T1i with Canon 100mm f/2.8L USM macro on 68mm of Kenko tubes, Canon Speedlite 580EX II with 4" x 5" Opteka diffuser at end of lens, 1/250 second at f/9 nominal (about f/26 effective at 1.9X magnification), E-TTL exposure.
Processed as Zerene Stacker DMap, no retouching.
The source images turned out to be shot out of order by depth, so I rearranged them to be in order before running DMap. Not sure how much I gained by doing that; it was mostly an experiment to see if focus order could be computed from scale. (It could.)
The first image is about 75% of the width of the original frame, so about 8.8 mm field width as shown here. The second image is resized to 50% of actual pixels. Because of the f/26 effective, there's not much detail below here, and even this is pretty strongly sharpened.
--Rik