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Playing with a dor beetle aka. praise of focus stacking
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eurythyrea



Joined: 25 Dec 2010
Posts: 123
Location: Budapest, Hungary

PostPosted: Sat Apr 09, 2011 2:06 am    Post subject: Playing with a dor beetle aka. praise of focus stacking Reply with quote

Let me show you what happens when an amateur entomologist mind meets the amateur focus stacker mind. The subject is a dor beetle or earth-boring dung beetle (Trypocopris vernalis). I used three lenses for this series: the MP-E 65 macro lens of Canon, the Mitutoyo BD Plan APO 10x microscope objective and the JML 21/3.5 lens. Click each one to see larger!

Ventral view of the first half of the body:

Canon MP-E 65/2.8 @ 2x - lit with two LED lamps, diffused with paper cylinder. 74 shots in PMax.

Dorsal view of the second half of the body:

Canon MP-E 65/2.8 @ 2,5x - lit with two LED lamps, diffused with paper cylinder. 118 shots in PMax.

The left side protibia and protarsus and mesotibia:

JML 21/3.5 @ 5x - 580EXII flash + paper cylinder. 123 shots in PMax.

Top of the left metatibia and first segments of metatarsus:

Mitutoyo BD Plan APO 10x @ 10x - 580EXII flash 3 paper cylinder. 159 shots in 8 parts in PMax.

The left side metatarsus:

Mitutoyo BD Plan APO 10x @ 11x - 580EXII flash 3 paper cylinder. 121 shots in PMax.

Cross your eyes carefully:


Rock baby!


Have a nice weekend!
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Nikola Rahmé

Hungary

http://www.flickr.com/photos/eurythyrea/


Last edited by eurythyrea on Sat Apr 09, 2011 3:14 am; edited 1 time in total
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seta666



Joined: 19 Mar 2010
Posts: 866
Location: Castellon, Spain

PostPosted: Sat Apr 09, 2011 2:52 am    Post subject: Reply with quote

Wonderfull series!!! framing, lighting... everything is perfect
Love the Dorsal view!!
Regards
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www.macrosmuymacros.com
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Craig Gerard



Joined: 01 May 2010
Posts: 2877
Location: Australia

PostPosted: Sat Apr 09, 2011 5:00 am    Post subject: Reply with quote

Nikola,

Outstanding detail and lighting!

A lot of work, some fine glass and excellent results. All worth it! Smile



Craig
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rjlittlefield
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Joined: 01 Aug 2006
Posts: 19783
Location: Richland, Washington State, USA

PostPosted: Sat Apr 09, 2011 8:53 am    Post subject: Reply with quote

Briefly: Shocked Very Happy

These are astonishing (and gratifying!).

I almost hate to ask, but how much time do you spend retouching these?

--Rik
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eurythyrea



Joined: 25 Dec 2010
Posts: 123
Location: Budapest, Hungary

PostPosted: Sun Apr 10, 2011 12:19 pm    Post subject: Reply with quote

rjlittlefield wrote:
These are astonishing (and gratifying!).
I almost hate to ask, but how much time do you spend retouching these?


Thank you Rik! Yes, I spend most of the time with retouching making a picture like these. It is much more than taking the exposures and stacking them in Zerene. Let's say 3-4 hours per one image.
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Nikola Rahmé

Hungary

http://www.flickr.com/photos/eurythyrea/
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Perel



Joined: 20 Mar 2011
Posts: 11

PostPosted: Sun Apr 10, 2011 3:11 pm    Post subject: Reply with quote

Great series and great Flickr too. Congratulations.

How do you do the Rolling Image? I would like to learn that, hehe

Thanks
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DrLazer



Joined: 16 Feb 2011
Posts: 293

PostPosted: Mon Apr 11, 2011 3:23 am    Post subject: Reply with quote

wonderful images Nikola
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eurythyrea



Joined: 25 Dec 2010
Posts: 123
Location: Budapest, Hungary

PostPosted: Mon Apr 11, 2011 11:51 am    Post subject: Reply with quote

Perel wrote:
Great series and great Flickr too. Congratulations.

How do you do the Rolling Image? I would like to learn that, hehe

Thanks


Hello Pedro! You can learn it on Zerene Satcker itself. Visit here:

http://www.zerenesystems.com/stacker/docs/SyntheticStereo.php

Nikola
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Nikola Rahmé

Hungary

http://www.flickr.com/photos/eurythyrea/
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pierre



Joined: 04 Jan 2010
Posts: 246
Location: France, Var, Toulon

PostPosted: Fri Apr 15, 2011 12:25 pm    Post subject: Reply with quote

Impressive Nikola Cool
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Pierre
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Mitch640



Joined: 15 Aug 2010
Posts: 2137

PostPosted: Fri Apr 15, 2011 12:39 pm    Post subject: Reply with quote

I see an effect that focus stacking seems to add to the chitin, not just on these shots, but all focus stacked images by anybody, so it's not specific to one person or technique. If you look at the sections in #5 you see they look like they are made of dark amber. I have seen something similar before, and just couldn't put my finger on it.

Now I remember. It looks just like tortoise shell. I once had a real tortoise shell comb, and the material looked just like this chitin. Is chitin and shell made of the same stuff?
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Marpee



Joined: 09 Nov 2010
Posts: 23
Location: Italy

PostPosted: Sat Apr 23, 2011 12:12 am    Post subject: Reply with quote

Great images. They are perfect, clean in every detal. How can you obtein that foreground? Thank you.

Congratulations.

Marco
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rjlittlefield
Site Admin


Joined: 01 Aug 2006
Posts: 19783
Location: Richland, Washington State, USA

PostPosted: Sat Apr 23, 2011 12:58 am    Post subject: Reply with quote

Mitch640 wrote:
It looks just like tortoise shell. I once had a real tortoise shell comb, and the material looked just like this chitin. Is chitin and shell made of the same stuff?

Very different chemistry, somewhat similar physical structure and function. I think tortoise shell is mostly keratin, which is a family of proteins -- a bunch of amino acids strung together in a line. Chitin is more like a polysaccharide -- a bunch of modified sugars strung together in a line. See http://en.wikipedia.org/wiki/Keratin and http://en.wikipedia.org/wiki/Chitin. But yeah, they both have that "dark amber" appearance you mentioned, due to absorbing quite a bit of light (especially blue) but not scattering very much.

--Rik
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Mitch640



Joined: 15 Aug 2010
Posts: 2137

PostPosted: Sat Apr 23, 2011 2:06 am    Post subject: Reply with quote

Very interesting. I learned something useful again. Smile
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Martin G.



Joined: 25 Dec 2010
Posts: 113
Location: Montreal, QC, Canada

PostPosted: Sat Apr 23, 2011 6:25 pm    Post subject: Reply with quote

absolutely stunning pictures, thanks for sharing!
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Cyclops



Joined: 05 Aug 2006
Posts: 2968
Location: North East of England

PostPosted: Sun Apr 24, 2011 2:44 am    Post subject: Reply with quote

Fabulous images!
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