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Tiny focus steps: how to make them, how to measure them
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rjlittlefield
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Joined: 01 Aug 2006
Posts: 20494
Location: Richland, Washington State, USA

PostPosted: Wed Nov 24, 2010 11:07 pm    Post subject: Reply with quote

ChrisLilley wrote:
scitch wrote:
It looks like your microscope stage is moving vertically, but your camera is mounted horizontally. What am I missing?

I believe that what you are missing is that this is not a focus stacking setup.

Its a distance-measuring setup, which happens to use focus stacking software (or rather, the image alignment part of the focus stacking software).

Correct. If it were being a focus stacking setup at the same time, there would be a second camera mounted above the microscope, in the spirit of THIS SETUP. But since the second camera would not affect the stage movements, I didn't bother to install one for these tests.

--Rik
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