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fern spore capsules

 
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Charles Krebs



Joined: 01 Aug 2006
Posts: 5805
Location: Issaquah, WA USA

PostPosted: Tue Jul 06, 2010 7:26 pm    Post subject: fern spore capsules Reply with quote

25X (with 10X M SPlan) and 50X (with 20X M SPlan) on sensor. Olympus BHA with BH2-UMA vertical illuminator and NFK 2.5X photo-eyepiece. Brightfield with crossed polarizers to reduce glare.





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Craig Gerard



Joined: 01 May 2010
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Location: Australia

PostPosted: Tue Jul 06, 2010 8:16 pm    Post subject: Reply with quote

Charlie,

The choice of objectives and photo-eyepiece. Was that due to working distance requirements and/or other additional factors?

Looking forward to seeing what happens next with these fern spores.


Craig
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Charles Krebs



Joined: 01 Aug 2006
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Location: Issaquah, WA USA

PostPosted: Tue Jul 06, 2010 8:28 pm    Post subject: Reply with quote

The choice was simply to get the composition I wanted.

I prefer to use the 1.67X NFK, but that is usually in the BHS stand. If I am going to take a number of shots with the BHA and epi illumination, I will switch it out. If, like in this case it is simply a single subject with a couple shots I'll sometimes just leave the 2.5X in, as I have done here.
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sonyalpha



Joined: 24 Apr 2010
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Location: Middle England

PostPosted: Wed Jul 07, 2010 12:19 am    Post subject: Reply with quote

Amazing........these look animal rather that vegetable:

What wonderful colour and texture too:

sonyalpha
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discomorphella



Joined: 01 Oct 2006
Posts: 605
Location: NW USA

PostPosted: Thu Jul 08, 2010 8:53 pm    Post subject: Reply with quote

Spectacular. That's a great use of an epi illuminator. I must admit these remind me of the clutch of eggs from one of the Alien movies. Now I have to go and grab a fern from the woods, what species did you use?

Regards,

David
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Charles Krebs



Joined: 01 Aug 2006
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Location: Issaquah, WA USA

PostPosted: Thu Jul 08, 2010 9:15 pm    Post subject: Reply with quote

David,

Pretty sure this is a Western sword fern (Polystichum munitum). It is a different species than the "macro" shot I posted over in the "Technical and Studio" section. This time of year there are many fern species that offer very interesting shots of sori and the sporangia.
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Julian Brooks



Joined: 14 Jun 2010
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Location: Oxfordshire, UK

PostPosted: Fri Jul 09, 2010 12:58 am    Post subject: Reply with quote

wow, the lighting on the second pic is spectacular. They remind me of cornish pasties, and somehow the pictures bring out the tangible living quality of these spores. Could you explain how crossing polarizers reduces glare, since the reflections from the surface will only go through one of them? Brilliant as ever.

Jules
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sonyalpha



Joined: 24 Apr 2010
Posts: 916
Location: Middle England

PostPosted: Fri Jul 09, 2010 1:06 am    Post subject: Reply with quote

Julian Brooks wrote:
wow, the lighting on the second pic is spectacular. They remind me of cornish pasties, and somehow the pictures bring out the tangible living quality of these spores. Could you explain how crossing polarizers reduces glare, since the reflections from the surface will only go through one of them? Brilliant as ever.

Jules


Hi Jules,

Being a fellow Brit......I can see what you mean about looking like Cornish Pasties..............but....but........do our American cousins on here know what Cornish Pasties are???

P . S . If you don't know Google them: Wink

sonyalpha
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Charles Krebs



Joined: 01 Aug 2006
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Location: Issaquah, WA USA

PostPosted: Fri Jul 09, 2010 2:14 pm    Post subject: Reply with quote

Jules,

Quote:
Could you explain how crossing polarizers reduces glare, since the reflections from the surface will only go through one of them?


Because the subject is being illuminated by light that has already polarized.

The light illuminating the subject is polarized by "A" in the first diagram below. The image forming light reflected from the subject then passes through the second polarizer "B" ("analyzer") before reaching the sensor. Polarizer "A" has a rotating mount, so when rotated it controls the glare off the subject. Direct "epi" brightfield is a rather "static" direct frontal lighting, and sometimes it's tough to get pleasing effects. But without the polarizers the glare is really hard to deal with.




Light comes in at "5". Is polarized by pol "A". Hits half-mirror unit at "3", is reflected down onto subject. Light reflected from subject passes upward through pol "B" and on to eyepieces and camera.


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Ecki



Joined: 13 Aug 2008
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Location: Cape Town, South Africa

PostPosted: Fri Jul 09, 2010 2:22 pm    Post subject: Reply with quote

I have never seen it like this.

Chapeou, Charles!
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rjlittlefield
Site Admin


Joined: 01 Aug 2006
Posts: 20070
Location: Richland, Washington State, USA

PostPosted: Fri Jul 09, 2010 6:15 pm    Post subject: Reply with quote

Julian Brooks wrote:
Could you explain how crossing polarizers reduces glare, since the reflections from the surface will only go through one of them?

The key detail is that specular reflections retain whatever polarization the illumination had, while non-specular reflections do not. Hence the polarizer on the lens, crossed against the illumination, can almost completely kill specular reflections. while leaving mostly intact the rest of the image content. See http://www.photomacrography.net/forum/viewtopic.php?t=3945 for full discussion.

--Rik
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Simon W



Joined: 01 Jul 2011
Posts: 153
Location: Melbourne, Australia

PostPosted: Fri Jul 15, 2011 8:30 pm    Post subject: Reply with quote

Charles, as I trawl through the forums, I keep coming across your helpful posts, but more importantly, some pretty stunning images. I really loved this one of yours, the colours, focus, everything. Enough said.
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