Bacteria ?

Images made through a microscope. All subject types.

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Jan l'Amie
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Bacteria ?

Post by Jan l'Amie »

Those different types of tiny organisms I found next to a dead waterflea.
Are they all bacteria ?

Canon 50D
2.5x Photo oculair
Plan 40/0.65 Plan 25/0.40
Kreutz filter

Image

Image

Image

Image
Byomic BYO500T microscope /CIOC XDS-1 invert.
Byomic ST-340 stereomicroscope
Olympus BHM Metallurgical Microscope
Canon EOS 7D Mark II / Canon MP-E65
Cognisys StackShot
www.ngvm.nl

olivier barth
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Location: FRANCE

Post by olivier barth »

Hi Jan
the biggest are probably protozoa. the thin rod-shaped ones are likely bacteria (bacillus).

Jan l'Amie
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Joined: Tue Jul 26, 2011 11:32 pm
Location: The Netherlands
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Post by Jan l'Amie »

O.K. Thanks very much for the reaction.
Byomic BYO500T microscope /CIOC XDS-1 invert.
Byomic ST-340 stereomicroscope
Olympus BHM Metallurgical Microscope
Canon EOS 7D Mark II / Canon MP-E65
Cognisys StackShot
www.ngvm.nl

Franz Neidl
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Joined: Wed Jan 14, 2009 11:59 am
Location: Italy

Post by Franz Neidl »

I would not exclude that the bigger ones are bacteria. The bacterium Achromatium oxaliferum is 5 to 100 µm long. Therefore it would be good if you make also some measurements!

Franz

Mitch640
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Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

Nice images Jan, your oblique lighting is very even and clear.

Jan l'Amie
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Joined: Tue Jul 26, 2011 11:32 pm
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Post by Jan l'Amie »

Thanks. At the 3th picture you see the desmids Micrasterias crux-melitensis and Micrasterias thomasiana. Those are about 100 and 250 micron. So the biggest organisms are about 20 to 60 micron.
I think it is Achromatium oxaliferum. They look exactly the same as pictures at the internet.

For oblique light I use only my cheap and very easy to make home-made Kreutz filter.
Byomic BYO500T microscope /CIOC XDS-1 invert.
Byomic ST-340 stereomicroscope
Olympus BHM Metallurgical Microscope
Canon EOS 7D Mark II / Canon MP-E65
Cognisys StackShot
www.ngvm.nl

Jan l'Amie
Posts: 268
Joined: Tue Jul 26, 2011 11:32 pm
Location: The Netherlands
Contact:

Post by Jan l'Amie »

A better picture.

Image
Byomic BYO500T microscope /CIOC XDS-1 invert.
Byomic ST-340 stereomicroscope
Olympus BHM Metallurgical Microscope
Canon EOS 7D Mark II / Canon MP-E65
Cognisys StackShot
www.ngvm.nl

Cactusdave
Posts: 1631
Joined: Tue Jun 09, 2009 12:40 pm
Location: Bromley, Kent, UK

Post by Cactusdave »

That's a fascinating bacterium. Never seen anything like it. It must be as big as a decent sized ciliate, and you can clearly resolve some structure inside the cells. You are squeezing some really nice results from your setup. The low power photo ocular you have is a real bonus in a setup like this.
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

Mitch640
Posts: 2137
Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

I would like to see a picture of your "very easy to make home-made Kreutz filter." Getting good oblique lighting on my scope is like pulling teeth.

Jan l'Amie
Posts: 268
Joined: Tue Jul 26, 2011 11:32 pm
Location: The Netherlands
Contact:

Post by Jan l'Amie »

I read something about this bacteria and it is indeed a very special thing.
Now and then you see one in a sample but I've never seen so many.

This is a Kreutz filter. I made it from a piece of transparant acrylat foil with on that a piece of black sticky foil. Whit a sharp knife you have to cut a moon-shaped segment out of the black foil with the size of about 1/3 of the filter size. The diafragma whole open and you have oblique light.
Maybe you have to make another one if you not satisfied with the first result.

Image
Byomic BYO500T microscope /CIOC XDS-1 invert.
Byomic ST-340 stereomicroscope
Olympus BHM Metallurgical Microscope
Canon EOS 7D Mark II / Canon MP-E65
Cognisys StackShot
www.ngvm.nl

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