DIC + epifluorescence

bromodomain · Post by **bromodomain** » Thu Jul 14, 2011 11:03 am

This is one of my first attempts at DIC ever and since I was missing parts I had to improvise so I used a circular polarizer with a λ/4 compensator in conjunction with fluorescence. Thats a rat nerve that I cut and I think this is the zone of myelin transition between the central and peripheral nervous systems.

Unfortunately the camera wasn't working properly and I was acquiring images with a different exposure than the preview one

DNA stained with DAPI

I used an optigrid panel to spatially attenuate the UV beam and thus managed to illuminate only half the field. You can see the birefringent Schwann cells and the difference in DNA (or cellular density) across the transition.

I wish I had the 20x/0.75 Planapo nomarski prism because this is my favourite objective

This image was acquired with a 10x/0.3 Plan-NEOFLUAR.

Cactusdave · Post by **Cactusdave** » Fri Jul 15, 2011 6:00 am

Looks like you may have some interesting optical effects going on there. I'd like to see some more pictures using the circular polariser and quarter wave compensator.

bromodomain · Post by **bromodomain** » Fri Jul 15, 2011 3:02 pm

deleted

bromodomain · Post by **bromodomain** » Sat Jul 16, 2011 3:59 pm

Cactusdave wrote:Looks like you may have some interesting optical effects going on there.

If I'm using circularly polarised light wouldn't that be C-DIC?