Just don't get around like I used to!
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- Charles Krebs
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Just don't get around like I used to!
That is even a common copepod lament!
You know how it goes... first comes the family, then you put on a few extra pounds of Epistylus... then those pesky little euglena...
And one other subject from yesterdays slide, Campanella umbellaria. Not too much interesting new activity in my local sampling pond. But there is always something to see.
You know how it goes... first comes the family, then you put on a few extra pounds of Epistylus... then those pesky little euglena...
And one other subject from yesterdays slide, Campanella umbellaria. Not too much interesting new activity in my local sampling pond. But there is always something to see.
- twebster
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Goodness gracious, Charlie. These are wonderful images! I especially like #2. I can see how a copepod would be slowed down by all the hangers-on! These are wonderful images.
Tom Webster
Phoenix "The Valley of the Sun", Arizona, USA
The worst day photographing dragonflies is better than the best day working!
Phoenix "The Valley of the Sun", Arizona, USA
The worst day photographing dragonflies is better than the best day working!
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Those are really stunning images Charles
Linden
Linden
Linden Gledhill http://www.flickr.com/photos/13084997@N03/
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- Charles Krebs
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Thanks all!
Franz,
The first darkfield shot was a quick "snap" taken with the 4X meant primarily to provide a reference to what is seen in the second image.
Franz,
Yes the second shot was with the 10/0.40 S Plan Apo. Oblique brightfield. I used the NFK 1.67X (but the Olympus ntermediate piece provides an additional 1.25X, so it is at 21X on sensor.I suppose you used for it the objective 10x, the projection eyepiece ?
The first darkfield shot was a quick "snap" taken with the 4X meant primarily to provide a reference to what is seen in the second image.
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Thank you Charles,
your answer is very interesting for me. I thought the second picture was with DIC. Now I have two questions:
1. How do you make a oblique brightfield?
2. You use an Olympus 1,67 projection eyepiece. I have only a 2,5 Nikon projection-eyepiece. It is possible to use an Olympus 1,67 eyepiece on a Nikon microscope? (I want to reduce the magnification of my microscope. Nikon does not have a 1,67 eyepiece and the Nikon 2,0 eyepiece is very expensive. What can I do?).
Franz
your answer is very interesting for me. I thought the second picture was with DIC. Now I have two questions:
1. How do you make a oblique brightfield?
2. You use an Olympus 1,67 projection eyepiece. I have only a 2,5 Nikon projection-eyepiece. It is possible to use an Olympus 1,67 eyepiece on a Nikon microscope? (I want to reduce the magnification of my microscope. Nikon does not have a 1,67 eyepiece and the Nikon 2,0 eyepiece is very expensive. What can I do?).
Franz
- Wim van Egmond
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- Charles Krebs
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Franz,
The effect varies greatly and can be extremely nice and effective. I used it extensively before I got DIC, and, in some cases I still prefer it to DIC.
2) The Olympus 1.67 NFK is no longer made and it has gotten gotten very expensive over the past few years (typically $600 and up). But it was "corrective" and would therefore not be a good choice with newer "infinity" microscopes that do not use correction in the eyepieces. What is the diameter of the tube where you now insert the 2.5X Nikon photo-eyepiece? What have you seen as a price for a 2X? Which camera are you using now?
1) I use the brightfield position of my "turret" condenser, but then rotate the turret slightly as if moving it to a different position. This moves the aperture off-center. Then I play around with the aperture size and the amount of decentering. Pulling an eyepiece and looking down at the back of the object will show something like these illustrations:1. How do you make a oblique brightfield?
2. You use an Olympus 1,67 projection eyepiece. I have only a 2,5 Nikon projection-eyepiece. It is possible to use an Olympus 1,67 eyepiece on a Nikon microscope? (I want to reduce the magnification of my microscope. Nikon does not have a 1,67 eyepiece and the Nikon 2,0 eyepiece is very expensive. What can I do?).
The effect varies greatly and can be extremely nice and effective. I used it extensively before I got DIC, and, in some cases I still prefer it to DIC.
2) The Olympus 1.67 NFK is no longer made and it has gotten gotten very expensive over the past few years (typically $600 and up). But it was "corrective" and would therefore not be a good choice with newer "infinity" microscopes that do not use correction in the eyepieces. What is the diameter of the tube where you now insert the 2.5X Nikon photo-eyepiece? What have you seen as a price for a 2X? Which camera are you using now?
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Hello Charles,
thank you very much for your answer.
To point 1: Decenter in the indicated way is an excellent idea. I will try it.
To point 2: My microscope is a Nikon “infinity” microscope. If the 1,67 NFK from Olympus is corrected and expensive it would not have sense for me to buy it.
The diameter of the 2,5 Nikon eyepiece (which I use now) is 30 mm.
The price for the Nikon PLI-2x photo eyepiece is 700 Euro (about 980 american dollars). In the moment (and maybe in the next future) this price is too high for me.
The camera I use now is a Pentax K20D (with factor 1,5x of magnification).
The reason why I want to reduce the magnification is because the DIC-objectives are starting with the objective 10x and sometimes I have very “big” objects like animals from the plankton which I want to photograph with DIC but they are simply to big for objective 10x.
Franz
thank you very much for your answer.
To point 1: Decenter in the indicated way is an excellent idea. I will try it.
To point 2: My microscope is a Nikon “infinity” microscope. If the 1,67 NFK from Olympus is corrected and expensive it would not have sense for me to buy it.
The diameter of the 2,5 Nikon eyepiece (which I use now) is 30 mm.
The price for the Nikon PLI-2x photo eyepiece is 700 Euro (about 980 american dollars). In the moment (and maybe in the next future) this price is too high for me.
The camera I use now is a Pentax K20D (with factor 1,5x of magnification).
The reason why I want to reduce the magnification is because the DIC-objectives are starting with the objective 10x and sometimes I have very “big” objects like animals from the plankton which I want to photograph with DIC but they are simply to big for objective 10x.
Franz
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- arturoag75
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