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Denizens of the (not very) deep!
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Charles Krebs



Joined: 01 Aug 2006
Posts: 5805
Location: Issaquah, WA USA

PostPosted: Sat May 22, 2010 10:52 pm    Post subject: Denizens of the (not very) deep! Reply with quote

Here are a few shots from the salt water sample I took this week from a fishing pier in Seattle.

4/0.16 S Plan Apo, brightfield. Not a very large "stack", but it drove me crazy moving the little "plumes". I gave up and was later surprised that there was one batch that looked OK.


60/1.40 S Plan Apo, DIC. This dinoflagellate was really far too "deep" into the slide for the 60X. I could barely focus without hitting the cover slip. As a result the resolution isn't very good, but it was an interesting angle on a "dino" I had not seen before.


10/0.40 S Plan Apo, darkfield. No idea what this is... (but it looks neat!)


20/0.46 S Plan Achromat, darkfield. I can't do darkfield with the 20/0.70 without changing condensers, so I occasionally put a 0.46 Plan Achromat on the nosepiece for times when the 10X just isn't enough.


10/0.40 S Plan Apo, darkfield. Cropped from the center of a horizontal frame.


This was an old 25X Zeiss Neofluar (0.60 I think). When used with the darkfield stop I've placed in my condenser it provides a lighting that is right on the "edge" of darkfield and "circular oblique".
It doesn't always look that good, but with certain subjects it seems to work nicely. This was a very deep slide and these diatoms were right up against the cover slip. The other debris had settled to the bottom and made what I felt was an interesting background


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sonyalpha



Joined: 24 Apr 2010
Posts: 916
Location: Middle England

PostPosted: Sun May 23, 2010 12:39 am    Post subject: Reply with quote

Charles.......these are just amazing:

I don't do this kind of photography but I really appreciate the technical and artistic effort that goes in to producing such work:

I can appreciate these on more that one level...........the one thought striking me at present is that ........what fantastic design ideas for a set of very classy jewellery you have here..........show them to a gold/silversmith as soon as you can:

To see these tiny creatures brings home just how devastating oil-spills and pollution can be:

Thank you for posting:

P.S. I will leave all the technicalities and the Latin names to the relevant experts:Very Happy

sonyalpha
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fpelectronica



Joined: 19 Feb 2010
Posts: 1808
Location: España

PostPosted: Sun May 23, 2010 1:35 am    Post subject: Reply with quote

Hello Charles:

Very nice shots!!!
Fascinating and enigmatic salt water organism.

Best regards.
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Tardigrade37



Joined: 04 Mar 2008
Posts: 134

PostPosted: Sun May 23, 2010 10:52 am    Post subject: Reply with quote

Hi Charles,

Beautiful as always!

I do have one short question - what is the source of the horizontal lines in the background of the first image?

Regards,

Chris
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Charles Krebs



Joined: 01 Aug 2006
Posts: 5805
Location: Issaquah, WA USA

PostPosted: Sun May 23, 2010 12:33 pm    Post subject: Reply with quote

What lines? Wink Wink (not fair, I know... they are gone now)

Actually I don't know! I went back to the original source files, the completed stack, the color corrected/edited "TIF" version I had saved and don't see a hint of them. I checked the "jpg" file that was "saved for web" in the folder that I use to upload to my site for the forum links, and this file is also without problems. So it happened somewhere from the point where I started to upload it. I'm not computer savvy enough to know how that could happen, but the only explanation I can see now is that it somehow was "corrupted" in the uploading process. I've never noticed it before.

edit... I've uploaded and replaced the image several times now. On a couple of occasions it showed these faint horizontal bands, and other times not! So it does appear to be an "uploading" problem.
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rjlittlefield
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Joined: 01 Aug 2006
Posts: 20082
Location: Richland, Washington State, USA

PostPosted: Sun May 23, 2010 1:07 pm    Post subject: Reply with quote

My first thought after hearing "upload" and "corruption" was that there had been a glitch in one of the tables that JPEG uses to do its last bit of compression. That sort of error could conceivably propagate from being a small localized error in a lookup table to being a globally visible repeating pattern in the image.

It's odd that the problem is even semi-reproducible. Usually file corruption during upload is completely random. Puzzling... Confused

--Rik
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Cyclops



Joined: 05 Aug 2006
Posts: 2968
Location: North East of England

PostPosted: Sun May 23, 2010 1:47 pm    Post subject: Reply with quote

Lovely shots Rik, that first one is a real oddity,love it!
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rjlittlefield
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Joined: 01 Aug 2006
Posts: 20082
Location: Richland, Washington State, USA

PostPosted: Sun May 23, 2010 1:50 pm    Post subject: Reply with quote

Cyclops wrote:
Lovely shots Rik, that first one is a real oddity,love it!

Thanks, but the pictures are from Charles. This sort of photography is far beyond my capabilities in every regard!

--Rik
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Franz Neidl



Joined: 14 Jan 2009
Posts: 747
Location: Italy

PostPosted: Sun May 23, 2010 1:58 pm    Post subject: Reply with quote

Hallo Charles!

Congratulation! The third picture could show a larva (maybe a Dipleurula) from Asterias rubens (commion starfish) and the last picture Ditylum brightwellii.

Franz
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Cyclops



Joined: 05 Aug 2006
Posts: 2968
Location: North East of England

PostPosted: Sun May 23, 2010 2:34 pm    Post subject: Reply with quote

rjlittlefield wrote:
Cyclops wrote:
Lovely shots Rik, that first one is a real oddity,love it!

Thanks, but the pictures are from Charles. This sort of photography is far beyond my capabilities in every regard!

--Rik

lol, oh dear! Its the heat today, finally got to me!
Great shots Charles!
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lauriek
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Joined: 25 Nov 2007
Posts: 2404
Location: South East UK

PostPosted: Sun May 23, 2010 3:56 pm    Post subject: Reply with quote

Fantastic set Charlie, I particularly like the last two..

...and I second Sonyalpha's suggestion re jewellery, not sure how the heck you could make them but perhaps Fabergé or someone could do them justice. Smile
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ScottH



Joined: 07 Feb 2010
Posts: 33

PostPosted: Sun May 23, 2010 7:12 pm    Post subject: Reply with quote

Wow again - great images - please keep adding these !

Scott H.
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RogelioMoreno



Joined: 20 Nov 2009
Posts: 2962
Location: Panama

PostPosted: Sun May 23, 2010 8:58 pm    Post subject: Reply with quote

Charles,

Amazing!

Rogelio
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svalley



Joined: 03 Dec 2006
Posts: 308
Location: Albany, Oregon

PostPosted: Fri May 28, 2010 2:30 pm    Post subject: Reply with quote

Wow! Charlie, these are stunning!

Steve
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PaulFurman



Joined: 24 Oct 2009
Posts: 595
Location: SF, CA, USA

PostPosted: Thu Jun 03, 2010 8:30 pm    Post subject: Reply with quote

Wow. I'd barely stumbled into this group before, this is inspiring!
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