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Ant Eyes

 
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RogelioMoreno



Joined: 20 Nov 2009
Posts: 2962
Location: Panama

PostPosted: Mon Apr 19, 2010 10:28 pm    Post subject: Ant Eyes Reply with quote

I was testing a new 20X objective and my ping pong ball diffuser.

Nikon 10/0.3 WI 160/- (without the WI tipping), direct projection (I removed the trinocular head and put the camera on the Nikon Labophot stand), ping pong ball diffuser, single FO illuminator, 273 frames with 4um steps:



L Plan 20/0.4 00/0 with Vivitar 200mm M42 acting as tube lens (I removed the trinocular head and put the camera + Vivitar 200mm + M42 spacers on the Nikon Labophot stand), ping pong ball diffuser, single FO illuminator, 98 frames with 2um steps:


Comments are welcome.

Rogelio
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elf



Joined: 18 Nov 2007
Posts: 1386

PostPosted: Tue Apr 20, 2010 9:05 am    Post subject: Reply with quote

The results are quite good. How much post processing have you done to these?

The step distance seems kind of small. The 10X's DOF should be around 15um. Do you get any focus banding if you stack every other frame or every third frame?
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RogelioMoreno



Joined: 20 Nov 2009
Posts: 2962
Location: Panama

PostPosted: Tue Apr 20, 2010 10:02 am    Post subject: Reply with quote

Elf,

I did contrast adjustment and sharpener. I think that in the first image I should had done some brightness adjustment.

The formula that I am using to calcule DOF says 5.7um DOF for a 0.3 NA. I have to try to stack without some frames.

Rogelio
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RogelioMoreno



Joined: 20 Nov 2009
Posts: 2962
Location: Panama

PostPosted: Tue Apr 20, 2010 10:16 pm    Post subject: Reply with quote

The previous images were stacked with HF, here are the version stacked with ZS:







Rogelio
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rjlittlefield
Site Admin


Joined: 01 Aug 2006
Posts: 20455
Location: Richland, Washington State, USA

PostPosted: Tue Apr 20, 2010 11:09 pm    Post subject: Reply with quote

Rogelio,

Thanks for posting the comparison. I assume these are ZS PMax. I see the usual tradeoffs: halos are gone around edges but there is more bloom around bright reflections; structure is retained in out-of-focus regions, but there is more pixel noise especially in uniform background.

I also see irregular dust trails and some rotated streaking on edges, especially in the first image. Those artifacts usually mean that the ZS alignment algorithm has gotten confused by how things look as they go severely OOF.

For this sort of high magnification work, it is usually best to go into Options > Preferences > Alignment and turn off Scale and Rotation. I believe your focus mechanism moves straight up and down, so probably you can also turn off Shift X and Shift Y. Doing those things will eliminate the dust trails and streaking.

I am thinking the ZS user interface could use some improvement. Right now there is one set of default settings. Those work well at lower magnifications, but as we see here they can give odd results at higher mags, and it's never obvious to new users what is going on. I will need to think about this...thanks for the illustrations.

--Rik
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RogelioMoreno



Joined: 20 Nov 2009
Posts: 2962
Location: Panama

PostPosted: Wed Apr 21, 2010 3:59 am    Post subject: Reply with quote

Rik,

Thank you for the info, I will try that.

Rogelio
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