Correct. A typical 10X microscope objective at NA 0.25 - 0.30 is roughly equivalent to f/1.8-1.5 in an ordinary lens.bvalente wrote:on the diffraction side of things, if I understand microscope objectives it sounds like they have much larger relative apertures and therefore would suffer less diffraction.
It would be very helpful to see what you write down. One reasonable approach would be to just take f/5.6 as a given, since that's pretty close to optimal for most lenses. Combine that with the simple formula for DOF that I gave above (as TDF=), and in most cases you'll be within a factor of 2X or so.Getting back to my original question/post, it seems like the "basic" math and roughing things in is exactly what would be helpful, at least to me and perhaps others who are at least familiar with photography and the basics, and need a starting point. Maybe I will rough something together.
--Rik