Staining

[PeglegOS]

I'm just starting out, so don't laugh too hard. I want to try staining, but don't have any. Or so I thought. I read that fish ick medicine contains malachite green, a stain.

Soooooooo, I went to my fish tank and took a bottle out of my fish kit. I cut some apple on my microtome, placed the sections in a dish with the ick medicine and left it for an hour.

Took a section and rinsed it a few time, popped it on a slide with a coverslip. When I looked in the scope, it had dyed the cell walls to a point of high visibilty. Compared to a plain apple section, it was 200% improvement.

This staining stuff really works. Now to get some real stains.

[DaveW]

Malachite green can be toxic in certain circumstances and possibly tumor forming Pegleg so be careful and wash your hands well after touching it before eating.

See:-

http://en.wikipedia.org/wiki/Malachite_green

http://www.defra.gov.uk/news/latest/2002/malachite.htm

http://physchem.ox.ac.uk/MSDS/MA/malach ... oride.html

DaveW

[PeglegOS]

Thanks Dave. I use latex gloves, even when I use it in the tanks.

Malachite green can be toxic in certain circumstances and possibly tumor forming Pegleg so be careful and wash your hands well after touching it before eating.

See:-

http://en.wikipedia.org/wiki/Malachite_green

http://www.defra.gov.uk/news/latest/2002/malachite.htm

http://physchem.ox.ac.uk/MSDS/MA/malach ... oride.html

DaveW