Urania ripheus, Scales in transmitted light

[Guppy]

Hi

Some scales in transmitted light.

1


2


3


4


5


6


Camera: Nikon D810, full frame
Lens: NIKON M Plan, 60/0.7 ELWD, 210/0
ISO: 64
Exposure: transmitted light, 1 flash, YONGNUO YN560III
Shooting file format (RAW/JPG): RAW
Tripod: Reprostand
Species name: Chrysiridia rhipheus (Urania r.)
Stacking software / method: Zerene Stacker / PMax
Imaging scale: 60:1
Average stack step size (µm) with Cognisys StackShot: 0.5

Kurt

[Rikisub]

Excellent shots!

[rjlittlefield]

Very nice!

Images #1 and #2, are those isolated scales that have been arranged for display? I don't see any trace of wing membrane.

--Rik

[Guppy]

Hi Rik

I kept the wings in a wet can for 2 days.
Then pressed them between a glass plate and some flow paper.
After about 2 weeks carefully removed the wing from the glass plate,
some scales remained stuck on the glass plate.

Kurt

[rjlittlefield]

Interesting method of preparation. I would never have guessed!

--Rik

[Guppy]

Hi

To the information, here is an overview.
After 14 days, the scales are still flat and firm on the glass plate.
The pictures are not retouched and not cropped.


www.focus-stacking.ch/B/05388_00.JPG

Camera: Nikon D810
Lens: MITUTOYO M Plan Apo 10X/0.28 ∞/0 f=200
Tube lens: Raynox DCR-150
Number of stacking steps: 77
Average stack step size (µm) with Cognisys StackShot: 3


www.focus-stacking.ch/B/05389_00.JPG

Camera: Nikon D810
Lens: MITUTOYO M Plan Apo 10X/0.28 ∞/0 f=200
Tube lens: Raynox DCR-150
Number of stack steps: 96
Average stack step size (µm) with Cognisys StackShot: 1.5

Kurt