Asparagus, grape tomato, raspberry and iris seed pod

Charles Krebs · Post by **Charles Krebs** » Sat Apr 25, 2020 11:45 pm

Not my usual subject matter... but kind of fun!

Slices. Not super thin, or extremely small. Cut with a vibratome and photographed with a 4X and 10X.

This is a section of a "new" Iris flower seed pod. Two 4X frames stitched. Deliberately photographed in a "high key" manner.

Section of small grape tomato. Two 4X frames stitched.

Partial section of asparagus.

Cross section of raspberry drupelet. (One of the small individual units making up the raspberry).

Adalbert · Post by **Adalbert** » Sun Apr 26, 2020 1:17 am

very nice !

micro_pix · Post by **micro_pix** » Sun Apr 26, 2020 1:50 am

.

Wonderfully creative and beautifully executed!

David

Smokedaddy · Post by **Smokedaddy** » Sun Apr 26, 2020 11:10 am

... interesting subject.

WalterD · Post by **WalterD** » Tue Apr 28, 2020 3:38 am

Thanks for sharing these excellent pictures, lovely colour and detail.

You cut them without fixation on a vibratome? I suppose the tissue was strong enough to be sectioned?

Charles Krebs · Post by **Charles Krebs** » Mon May 04, 2020 1:38 am

Walter,

You cut them without fixation on a vibratome? I suppose the tissue was strong enough to be sectioned?

They were fresh subjects and were embedded in agarose which helped a great deal. Seeds were a problem with subjects like the "grape" tomato, because they would often get pushed through the soft tissue. These were not thin sections like you would cut with a microtome, About the thinnest I could cut with most subjects like this was about 50 micron, but with many subjects I used much thicker :slices". I could probably do that by hand with a razor blade, but they would not have the uniform thickness.

Ken Ramos · Post by **Ken Ramos** » Mon May 04, 2020 4:43 am

Excellent images and wonderful details in all of them Charles.