I bite you

Starting out in microscopy? Post images and ask questions relating to the microscope and get answers from our more advanced users on the subject.

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Luisifer
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I bite you

Post by Luisifer »


Luisifer
Posts: 84
Joined: Wed Sep 05, 2018 1:40 am
Location: Czech Republic
Contact:

Re: I bite you

Post by Luisifer »

Another one:

Image

(It should be Pholcus phalangioides)

Luisifer
Posts: 84
Joined: Wed Sep 05, 2018 1:40 am
Location: Czech Republic
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Re: I bite you

Post by Luisifer »

Third one is not a spider but beatle.

Carpophilus hemipterus (Linnaeus, 1758)

https://12in.cz/fotografie/obr/128__60_Lesknacek-fikovy

The simplest stack and stitch, two rows, first 199 frames and second 156 frames. But quite no simple stitching thanks to change in position of legs and antennae.

Image

cube-tube
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Post by cube-tube »

Incredible images! What gear are you using?

Luisifer
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Post by Luisifer »

For now i am using quite provisory setup of gear around the Mitutoyo 20x:

Image

---

But in slow progress is quite more precize three axis gear:

Image

On photo and on Z axis is not added planetary gearbox which should be here in future with ratio 100:1 and thanks to it 1k steps per 1 micron:
Image

ChrisR
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Post by ChrisR »

That's quite a set-up.
It's all on a tripod?! :shock:
I see ( ;) ) you have concrete floors, but I'm surprised there isn't vibration.

1000 steps per micron may be more than you need...
Chris R

Luisifer
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Post by Luisifer »

No, it is on second floor from planks and each bigger car cause vibrations or when i am not steady. :)

1000 steps per micron mean that i will use about 700 steps per second / between frames. I use permanent movement without stopping between each frame and i expect that more steps brings more fluence to movement than using less steps per second which are closer to move-stop-move-stop character.
(begin / end of movement can cause vibrations when momentum is changed)

cube-tube
Posts: 107
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Location: Durham, NC

Post by cube-tube »

Beautiful! That setup really made me smile.

Does this still count as beginner micro? :shock:

I'm having trouble figuring out your tube lens. Are you using multiple lenses? Whatever it is it seems to be working very well. The microcontrast around the hairs of the beetle is better than what I have achieved.

Luisifer
Posts: 84
Joined: Wed Sep 05, 2018 1:40 am
Location: Czech Republic
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Post by Luisifer »

I started with micro only about a year before. :-)

(that's why i use not appropriate gear yet .... but some again and again redoing of eL aluminium profiles are the case too)

Tube lens is: EOS/M58 + 2x M58 telescopic (adjustable) tubes + reductions to reversed DCR-150 + M42 tube + reductions from M42 to M26.

Image

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