VIS vs FL

Images made through a microscope. All subject types.

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MASZEK
Posts: 75
Joined: Wed Jul 04, 2018 2:14 am
Location: POLAND

VIS vs FL

Post by MASZEK »

Hey.

I like fluorescent effects due to interesting colors. And that's why I sometimes compare the results obtained with visible and fluorescent light. Small fragments of plants in the pictures.

Greetings

Image VIS
Image FL
Image VIS
Image FL
Image VIS
Image FL
Image VIS
Image FL

pbraub
Posts: 60
Joined: Fri Feb 02, 2018 1:23 pm

Post by pbraub »

Maszek, thank you for these impressive images - I can even get the stereo images to fuse in my head (which I have some troubles with usually).

Is the FL pure UV excitation or do you use multiple wavelengths?

Cheers
Peter

Sumguy01
Posts: 1516
Joined: Mon Jan 28, 2013 11:05 pm
Location: Ketchikan Alaska USA

Post by Sumguy01 »

:smt038 Very nice.
The stereo pairs really pop out.
Thanks for sharing.

iconoclastica
Posts: 277
Joined: Sat Jun 25, 2016 12:34 pm
Location: Wageningen, Gelderland

Post by iconoclastica »

Te occasions that I can get the stereo-pairs to fuse are rare and this is not one of them...

Macek, you write that you like the colours, but what I see (in the topmost pair) is that the subtle colours are traded for over-saturated reds.

Wim
--- felix filicis ---

Chris S.
Site Admin
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Joined: Sun Apr 05, 2009 9:55 pm
Location: Ohio, USA

Post by Chris S. »

Bravo, MASZEK! I also enjoyed viewing/comparing your images. My favorites are the two stereo pairs at the bottom of your post--the same plant part photographer under vis/fluorescent light.

Could you please provide the specifics for the fluorescent images (light source, filters, etc.)?

--Chris S.

ChrisR
Site Admin
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Joined: Sat Mar 14, 2009 3:58 am
Location: Near London, UK

Post by ChrisR »

I like these too.
The first has strong contrast, reds and blue/white. What plant is it? I'd like to try that one.
Perhaps another colour, or a little natural light, could fill the shadows a little.
Chris R

Jacek
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Location: Poland

Post by Jacek »

Very nice

carlos.uruguay
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Post by carlos.uruguay »

Nice!!

MASZEK
Posts: 75
Joined: Wed Jul 04, 2018 2:14 am
Location: POLAND

Post by MASZEK »

Dear

carlos.uruguay, Jacek, ChrisR, Chris S. iconoclastica, Sumguy01, pbraub.

I want to thank You very much for devoting your time and comments. Answering for your questions:

- I am neither biologist nor botanist. Therefore I have problem with specific name those organism which were photographed by me. I am treating it just like hobby. Microscopic slides I choose by accident. I am size slide up casually and if it is ok I handle it further.

By FL lighting I use excitation filter UG1, barrier filter PZOGG9, or PZOOG1+GG9. With the aim to generating stereopairs I am using ZS Programm. On the end of my work I use GIMP : light exposition + delicate improvement of background. I don't improve colours by computer.

Now I attach new photos .

Best regards


Image FL
Image FL
Image VIS
Image FL
Image VIS
Image FL

pbraub
Posts: 60
Joined: Fri Feb 02, 2018 1:23 pm

Post by pbraub »

Mazek,

thank you for the - again - wonderful photos and for the information on your setup!

Cheers
Peter

carlos.uruguay
Posts: 5353
Joined: Thu Feb 23, 2012 8:05 pm
Location: Uruguay - Montevideo - America del Sur
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Post by carlos.uruguay »

:smt038 :smt038

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