Images made through a microscope. All subject types.
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I did intend to use 405nm violet (no need for a cooled camera), but the experimental, prototype mountant I used turned out to be utterly opaque to violet (and cuts blue a bit too), hence white light for this one. The mountant has a higher R.I. than zrax but along with a few issues too. Great for high contrast in hi-res images though. The mountant was made by someone else, not me, so I'll leave them to comment further if they choose to.Lou Jost wrote:It's time for violet light and a cooled monochrome camera.....hehehe
Thanks to everyone else for your comments too.
Rik - sorry to say I deleted all the RAWs once I'd stacked the picture. It was just a test of the mountant really. I could shoot it again I guess, but perhaps you'll accept this one as a consolation. Same gear but a thicker diatom (75 micron FoV, 90-image stack) hence the extra in-focus crud.
A monochrome camera would be useful when using violet light, because it would have four active pixels where a Bayer-matrix sensor would have only two (and the red one might be noisy). Edit: Ah-- but you probably use pixel-shifting, which fixes that ....