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Beatsy
Joined: 05 Jul 2013
Posts: 1522
Location: Malvern, UK
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 Posted: Tue Nov 27, 2018 2:20 pm Post subject: Auliscus sp |
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Faffing with double immersion. Stack of 40 images, white light, DIC, 100/1.3 PlanAPO + 1.4 apl/achr condenser. Frustule is 50 microns diameter.
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Olympusman
Joined: 15 Jan 2012
Posts: 4169
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| Posted: Tue Nov 27, 2018 3:40 pm Post subject: Diatom |
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Impressive!
Mike
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Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA |
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rjlittlefield
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Joined: 01 Aug 2006
Posts: 19899
Location: Richland, Washington State, USA
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| Posted: Tue Nov 27, 2018 3:41 pm Post subject: |
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I'm not familiar with "double immersion". Can you say more?
--Rik |
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Beatsy
Joined: 05 Jul 2013
Posts: 1522
Location: Malvern, UK
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| Posted: Tue Nov 27, 2018 3:45 pm Post subject: |
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| Just meant both condenser and objective oiled to the slide. |
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Sumguy01
Joined: 28 Jan 2013
Posts: 1351
Location: Ketchikan Alaska USA
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| Posted: Tue Nov 27, 2018 9:21 pm Post subject: |
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 Very nice.
Thanks for sharing. |
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rjlittlefield
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Joined: 01 Aug 2006
Posts: 19899
Location: Richland, Washington State, USA
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| Posted: Tue Nov 27, 2018 11:23 pm Post subject: |
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| Beatsy wrote: | | Just meant both condenser and objective oiled to the slide. |
Got it, thanks.
You know, of course, that next I'm going to ask for stereo... 
--Rik |
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santiago
Joined: 25 Sep 2018
Posts: 108
Location: Nijmegen, The Netherlands
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| Posted: Wed Nov 28, 2018 12:00 am Post subject: |
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great work and fascinating diatom
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Santiago
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anne
Joined: 05 Dec 2014
Posts: 133
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| Posted: Wed Nov 28, 2018 3:51 am Post subject: |
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Hi Steve,
you know this is a really completely incrediable image?
Looks like SEM/REM in details.
Thank you for sharing.
Anne |
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Adalbert
Joined: 30 Nov 2015
Posts: 706
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| Posted: Wed Nov 28, 2018 4:11 am Post subject: |
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Hello Steve,
This is something special, extraordinary, I like it :-)
BR, ADi |
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santiago
Joined: 25 Sep 2018
Posts: 108
Location: Nijmegen, The Netherlands
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| Posted: Wed Nov 28, 2018 5:10 am Post subject: |
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it reminds of Ernst Haeckel illustrations
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Santiago
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Pau
Site Admin
Joined: 20 Jan 2010
Posts: 4790
Location: Valencia, Spain
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| Posted: Wed Nov 28, 2018 5:26 am Post subject: |
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I have an Auliscus in a prepared slide and can't get any close 
Now I'm ready to trow away my microscope and search a new hobby 
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Pau |
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Lou Jost
Joined: 04 Sep 2015
Posts: 3615
Location: Ecuador
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Beatsy
Joined: 05 Jul 2013
Posts: 1522
Location: Malvern, UK
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| Posted: Wed Nov 28, 2018 6:29 am Post subject: |
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| Lou Jost wrote: | | It's time for violet light and a cooled monochrome camera.....hehehe |
I did intend to use 405nm violet (no need for a cooled camera), but the experimental, prototype mountant I used turned out to be utterly opaque to violet (and cuts blue a bit too), hence white light for this one. The mountant has a higher R.I. than zrax but along with a few issues too. Great for high contrast in hi-res images though. The mountant was made by someone else, not me, so I'll leave them to comment further if they choose to.
Thanks to everyone else for your comments too.
Rik - sorry to say I deleted all the RAWs once I'd stacked the picture. It was just a test of the mountant really. I could shoot it again I guess, but perhaps you'll accept this one as a consolation. Same gear but a thicker diatom (75 micron FoV, 90-image stack) hence the extra in-focus crud.  |
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Lou Jost
Joined: 04 Sep 2015
Posts: 3615
Location: Ecuador
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| Posted: Wed Nov 28, 2018 6:35 am Post subject: |
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A monochrome camera would be useful when using violet light, because it would have four active pixels where a Bayer-matrix sensor would have only two (and the red one might be noisy). Edit: Ah-- but you probably use pixel-shifting, which fixes that ....
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Lou Jost
www.ecomingafoundation.wordpress.com
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santiago
Joined: 25 Sep 2018
Posts: 108
Location: Nijmegen, The Netherlands
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| Posted: Wed Nov 28, 2018 6:39 am Post subject: |
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great stereo, love the choice of depth, those 4 "mountains" are very high! 
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Santiago
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