Auliscus sp

Beatsy · Post by **Beatsy** » Tue Nov 27, 2018 2:20 pm

Faffing with double immersion. Stack of 40 images, white light, DIC, 100/1.3 PlanAPO + 1.4 apl/achr condenser. Frustule is 50 microns diameter.

Olympusman · Post by **Olympusman** » Tue Nov 27, 2018 3:40 pm

Impressive!

Mike

rjlittlefield · Post by **rjlittlefield** » Tue Nov 27, 2018 3:41 pm

I'm not familiar with "double immersion". Can you say more?

--Rik

Beatsy · Post by **Beatsy** » Tue Nov 27, 2018 3:45 pm

Just meant both condenser and objective oiled to the slide.

Sumguy01 · Post by **Sumguy01** » Tue Nov 27, 2018 9:21 pm

Very nice.
Thanks for sharing.

rjlittlefield · Post by **rjlittlefield** » Tue Nov 27, 2018 11:23 pm

Beatsy wrote:Just meant both condenser and objective oiled to the slide.

Got it, thanks.

You know, of course, that next I'm going to ask for stereo...

--Rik

santiago · Post by **santiago** » Wed Nov 28, 2018 12:00 am

great work and fascinating diatom

anne · Post by **anne** » Wed Nov 28, 2018 3:51 am

Hi Steve,
you know this is a really completely incrediable image?
Looks like SEM/REM in details.
Thank you for sharing.

Anne

Adalbert · Post by **Adalbert** » Wed Nov 28, 2018 4:11 am

Hello Steve,
This is something special, extraordinary, I like it :-)
BR, ADi

santiago · Post by **santiago** » Wed Nov 28, 2018 5:10 am

it reminds of Ernst Haeckel illustrations

Pau · Post by **Pau** » Wed Nov 28, 2018 5:26 am

I have an Auliscus in a prepared slide and can't get any close

Now I'm ready to trow away my microscope and search a new hobby

Lou Jost · Post by **Lou Jost** » Wed Nov 28, 2018 5:50 am

It's time for violet light and a cooled monochrome camera.....hehehe

Beatsy · Post by **Beatsy** » Wed Nov 28, 2018 6:29 am

Lou Jost wrote:It's time for violet light and a cooled monochrome camera.....hehehe

I did intend to use 405nm violet (no need for a cooled camera), but the experimental, prototype mountant I used turned out to be utterly opaque to violet (and cuts blue a bit too), hence white light for this one. The mountant has a higher R.I. than zrax but along with a few issues too. Great for high contrast in hi-res images though. The mountant was made by someone else, not me, so I'll leave them to comment further if they choose to.

Thanks to everyone else for your comments too.

Rik - sorry to say I deleted all the RAWs once I'd stacked the picture. It was just a test of the mountant really. I could shoot it again I guess, but perhaps you'll accept this one as a consolation. Same gear but a thicker diatom (75 micron FoV, 90-image stack) hence the extra in-focus crud.

Lou Jost · Post by **Lou Jost** » Wed Nov 28, 2018 6:35 am

A monochrome camera would be useful when using violet light, because it would have four active pixels where a Bayer-matrix sensor would have only two (and the red one might be noisy). Edit: Ah-- but you probably use pixel-shifting, which fixes that ....

santiago · Post by **santiago** » Wed Nov 28, 2018 6:39 am

great stereo, love the choice of depth, those 4 "mountains" are very high!

Auliscus sp

Auliscus sp

Diatom