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Auliscus sp
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Beatsy



Joined: 05 Jul 2013
Posts: 1522
Location: Malvern, UK

PostPosted: Tue Nov 27, 2018 2:20 pm    Post subject: Auliscus sp Reply with quote

Faffing with double immersion. Stack of 40 images, white light, DIC, 100/1.3 PlanAPO + 1.4 apl/achr condenser. Frustule is 50 microns diameter.
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Olympusman



Joined: 15 Jan 2012
Posts: 4173

PostPosted: Tue Nov 27, 2018 3:40 pm    Post subject: Diatom Reply with quote

Impressive!

Mike
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Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA
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rjlittlefield
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Joined: 01 Aug 2006
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Location: Richland, Washington State, USA

PostPosted: Tue Nov 27, 2018 3:41 pm    Post subject: Reply with quote

I'm not familiar with "double immersion". Can you say more?

--Rik
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Beatsy



Joined: 05 Jul 2013
Posts: 1522
Location: Malvern, UK

PostPosted: Tue Nov 27, 2018 3:45 pm    Post subject: Reply with quote

Just meant both condenser and objective oiled to the slide.
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Sumguy01



Joined: 28 Jan 2013
Posts: 1352
Location: Ketchikan Alaska USA

PostPosted: Tue Nov 27, 2018 9:21 pm    Post subject: Reply with quote

Very nice.
Thanks for sharing.
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rjlittlefield
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Joined: 01 Aug 2006
Posts: 19913
Location: Richland, Washington State, USA

PostPosted: Tue Nov 27, 2018 11:23 pm    Post subject: Reply with quote

Beatsy wrote:
Just meant both condenser and objective oiled to the slide.

Got it, thanks.

You know, of course, that next I'm going to ask for stereo... Angel

--Rik
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santiago



Joined: 25 Sep 2018
Posts: 108
Location: Nijmegen, The Netherlands

PostPosted: Wed Nov 28, 2018 12:00 am    Post subject: Reply with quote

great work and fascinating diatom
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Santiago
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anne



Joined: 05 Dec 2014
Posts: 133

PostPosted: Wed Nov 28, 2018 3:51 am    Post subject: Reply with quote

Hi Steve,
you know this is a really completely incrediable image?
Looks like SEM/REM in details.
Thank you for sharing.

Anne
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Adalbert



Joined: 30 Nov 2015
Posts: 706

PostPosted: Wed Nov 28, 2018 4:11 am    Post subject: Reply with quote

Hello Steve,
This is something special, extraordinary, I like it :-)
BR, ADi
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santiago



Joined: 25 Sep 2018
Posts: 108
Location: Nijmegen, The Netherlands

PostPosted: Wed Nov 28, 2018 5:10 am    Post subject: Reply with quote

it reminds of Ernst Haeckel illustrations
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Santiago
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Pau
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Joined: 20 Jan 2010
Posts: 4793
Location: Valencia, Spain

PostPosted: Wed Nov 28, 2018 5:26 am    Post subject: Reply with quote

I have an Auliscus in a prepared slide and can't get any close Evil or Very Mad
Now I'm ready to trow away my microscope and search a new hobby Laughing
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Pau
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Lou Jost



Joined: 04 Sep 2015
Posts: 3618
Location: Ecuador

PostPosted: Wed Nov 28, 2018 5:50 am    Post subject: Reply with quote

It's time for violet light and a cooled monochrome camera.....hehehe
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Lou Jost
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Beatsy



Joined: 05 Jul 2013
Posts: 1522
Location: Malvern, UK

PostPosted: Wed Nov 28, 2018 6:29 am    Post subject: Reply with quote

Lou Jost wrote:
It's time for violet light and a cooled monochrome camera.....hehehe


I did intend to use 405nm violet (no need for a cooled camera), but the experimental, prototype mountant I used turned out to be utterly opaque to violet (and cuts blue a bit too), hence white light for this one. The mountant has a higher R.I. than zrax but along with a few issues too. Great for high contrast in hi-res images though. The mountant was made by someone else, not me, so I'll leave them to comment further if they choose to.

Thanks to everyone else for your comments too.

Rik - sorry to say I deleted all the RAWs once I'd stacked the picture. It was just a test of the mountant really. I could shoot it again I guess, but perhaps you'll accept this one as a consolation. Same gear but a thicker diatom (75 micron FoV, 90-image stack) hence the extra in-focus crud.
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Lou Jost



Joined: 04 Sep 2015
Posts: 3618
Location: Ecuador

PostPosted: Wed Nov 28, 2018 6:35 am    Post subject: Reply with quote

A monochrome camera would be useful when using violet light, because it would have four active pixels where a Bayer-matrix sensor would have only two (and the red one might be noisy). Edit: Ah-- but you probably use pixel-shifting, which fixes that ....
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Lou Jost
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santiago



Joined: 25 Sep 2018
Posts: 108
Location: Nijmegen, The Netherlands

PostPosted: Wed Nov 28, 2018 6:39 am    Post subject: Reply with quote

great stereo, love the choice of depth, those 4 "mountains" are very high! Smile
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Santiago
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