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Thank you for the help

 
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garretgreen



Joined: 14 May 2018
Posts: 5
Location: CA

PostPosted: Mon Jun 18, 2018 1:47 am    Post subject: Thank you for the help Reply with quote

Thank you for the help with the sharp images issues I was having, now all is needed is a solid light system to get this thing running. Here is the flea i have been using to get this thing running! Also found out how to Photostack an image. Any ideas on how to get rid of 50 years of chips and gunk on the optics in the microscope.
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rjlittlefield
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Joined: 01 Aug 2006
Posts: 19327
Location: Richland, Washington State, USA

PostPosted: Thu Jun 21, 2018 9:57 pm    Post subject: Reply with quote

Glad to hear that you're getting sharper images now.

About cleaning the microscope, I assume the problem is that you're seeing dust & debris spots in your images. In that case, the first thing to do is figure out exactly where the gunk is. The simplest place to start is to rotate various pieces of the system, for example the eyepiece, condenser, and objective, each separately. Whichever one causes the spot to rotate along with the optics is where the offending debris is located.

Once you have found the debris, you can start to remove it, beginning with a blower, then a soft brush, then a swab wetted with distilled water. Be sure to wipe once, in one direction (or spiral), and do not re-use the swab. Some of the debris may be hard enough to scratch lenses, so use the swab in a way that will pick debris off the surface, not scrub it in. If you have debris that is not water-soluble, then you will have to use solvents, starting with say 70% ethanol. Be aware that some common glass-cleaning products such as Windex may contain ammonia, which may affect or remove lens coatings. Do a Google search on "cleaning microscope objectives" for good recommendations.

--Rik
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Pau
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Joined: 20 Jan 2010
Posts: 4498
Location: Valencia, Spain

PostPosted: Fri Jun 22, 2018 12:04 am    Post subject: Reply with quote

You can download the useful booklet "The clean microscope" from Zeiss

https://applications.zeiss.com/C125792900358A3F/0/3C1DAE76330E42F7C12579EC0028F56B/$FILE/50-1-0025_the_clean_microscope_e.pdf
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Pau
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garretgreen



Joined: 14 May 2018
Posts: 5
Location: CA

PostPosted: Mon Jun 25, 2018 5:02 am    Post subject: Reply with quote

With a little fear and some good old time I was able to get the microscope lens clean. Here is a Photo of an anther after I finished cleaning the optics. Very Happy I also got the layer stacking to run a bit better vs the first image.



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rjlittlefield
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Joined: 01 Aug 2006
Posts: 19327
Location: Richland, Washington State, USA

PostPosted: Mon Jun 25, 2018 7:39 am    Post subject: Reply with quote

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