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my first post

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Janak Choudhary

Joined: 28 Sep 2017
Posts: 1
Location: Jagadhri,Haryana,India

PostPosted: Sat Jun 09, 2018 8:58 am    Post subject: my first post Reply with quote

Posting here for the first time , would like to acknowledge what i gained from this forum. Thanx a lot this forum n all the Admins for providing in-depth knowledge of the subject . I learned a lot from the wonderful, cooperative persons n admin who really spared time to respond to the queries of all newbie . Thanx a lot all of u .
Sharing my work ,first image is of head shot of long legged fly .
10 x MAGNIFICATION,NIKON 10 x LENS, 25 micron step,77 pic stack,at Iso 200. Yongnou twin flash used as light source . a diy diffuser .
second shot is that of wasp , Mag.4X ,Step size 35 micron.stack of 108 images, f-5, lens canon MPE -65.macro twin flash used.
Most difficult part of learning was and is understanding light diffusion , still struggling .
second was achieving sharpness . My stacks are still not up to my satisfaction,particularly with Nikon 10 X . I have a stable table top studio set up . Table is quite heavy n on concrete floor, away from road. wemacro rail is mounted on heavy wooden base n i m using flash as light source with second curtain synch.
Please help me in achieving sharp pics .

I would like your valuable comments on two points ,
1- how to achieve sharpness , particularly with Nikon 10X objective, some of my stack were good or acceptable but others were hopeless .
2- lighting part .
I am newbie and learned macro from this forum only.
canon 1DX mk II .7DmkII,Canon 100mm macro,wemacro rail, nikon cfi 10X OBJECTIVE.Amscope 4X ,canon bellows FL,Raynox 250
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Joined: 11 Dec 2015
Posts: 16
Location: Vietnam

PostPosted: Thu Jun 21, 2018 8:19 pm    Post subject: Reply with quote

I think the main issue you've got is lighting. Sharpness is not too important here and thought you've got some vibration also.
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Site Admin

Joined: 01 Aug 2006
Posts: 18866
Location: Richland, Washington State, USA

PostPosted: Thu Jun 21, 2018 9:46 pm    Post subject: Reply with quote

Janak, welcome aboard! I apologize for the late greeting and response -- obviously I overlooked your first post.

Usually flash can freeze out whatever vibration there is, but for that it is important that the flash runs on low power, so you get a short flash. To accomplish this the diffuser should be placed close to the subject, so the flash can be close also. If you adjust the flash power by hand, 1/16 power or less should be enough to freeze out vibration.

It is also important to realize that a 10X NA 0.25 objective, at 10X on sensor, will be running at effective f/20 on sensor. This gives quite a lot of diffraction blur, particularly on smaller sensor like your 7D Mark II. As a result, your images out-of-camera will never look sharp when you look at actual pixels, 100% scale. To make them sharper, you can and should apply lots of digital sharpening. Because the original image has been softened so much by diffraction, you will need a level of sharpening that would cause obvious artifacts in an image that started sharper. I commonly use Photoshop's "unsharp mask" at 100% with radius 1 pixel, sometimes even more, when working with images shot through a microscope objective.

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