Hippeastrum flower pollen

Images made through a microscope. All subject types.

Moderators: ChrisR, Chris S., Pau, rjlittlefield

JH
Posts: 1277
Joined: Sat Mar 09, 2013 9:46 am
Location: Vallentuna, Stockholm, Sweden
Contact:

Hippeastrum flower pollen

Post by JH »

Hippeastrum flower pollen
Nikon ELWD 100x, Canon 6D, EPI-light, Zerene Stacker Adobe PS and LR, Topaz ReMask 5. I used quite a lot of sharpening, and blurred out the background.

Best regards
Jörgen Hellberg

Image

Image
Jörgen Hellberg, my webbsite www.hellberg.photo

rjlittlefield
Site Admin
Posts: 20867
Joined: Tue Aug 01, 2006 8:34 am
Location: Richland, Washington State, USA
Contact:

Post by rjlittlefield »

Impressive! How deep is the stack (frames & microns) ?

--Rik

Pau
Site Admin
Posts: 5106
Joined: Wed Jan 20, 2010 8:57 am
Location: Valencia, Spain

Post by Pau »

Excellent, the stereo helps a lot to see the actual shape of the pollen texture
Pau

JH
Posts: 1277
Joined: Sat Mar 09, 2013 9:46 am
Location: Vallentuna, Stockholm, Sweden
Contact:

Post by JH »

Thanks Rik and Pau!
rjlittlefield wrote:Impressive! How deep is the stack (frames & microns) ?

--Rik
The lens is NA 0.8 so usually a step size close to 0.0005 mm (0.5 microns) will be OK with a little marginal.

I was trying to do a depth map where the dents could be seen. To do this I had to have smaller than normal steps. In this case I used 0.0001 mm (0.1 micron), so 319 pictures covered the distance of approx. 0.032 mm (32 microns).


Best regards
Jörgen Hellberg
Jörgen Hellberg, my webbsite www.hellberg.photo

Jacek
Posts: 5353
Joined: Sun Oct 02, 2011 7:00 am
Location: Poland

Post by Jacek »

Good job, Very nice

Ken Ramos
Posts: 7176
Joined: Thu Jul 27, 2006 2:12 pm
Location: lat=35.4005&lon=-81.9841

Post by Ken Ramos »

So alien, so unearthly in appearance. Wonderful photograph!

JH
Posts: 1277
Joined: Sat Mar 09, 2013 9:46 am
Location: Vallentuna, Stockholm, Sweden
Contact:

Post by JH »

Thanks Ken Ramos and Jacek!
Best regards
Jörgen Hellberg
Jörgen Hellberg, my webbsite www.hellberg.photo

carlos.uruguay
Posts: 5354
Joined: Thu Feb 23, 2012 8:05 pm
Location: Uruguay - Montevideo - America del Sur
Contact:

Post by carlos.uruguay »

Nice!

Olympusman
Posts: 4543
Joined: Sun Jan 15, 2012 12:31 pm

Pollen

Post by Olympusman »

Lovely.

Mike
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

JH
Posts: 1277
Joined: Sat Mar 09, 2013 9:46 am
Location: Vallentuna, Stockholm, Sweden
Contact:

Post by JH »

Thanks Carlos and Mike.

Best regards
Jörgen Hellberg
Jörgen Hellberg, my webbsite www.hellberg.photo

JH
Posts: 1277
Joined: Sat Mar 09, 2013 9:46 am
Location: Vallentuna, Stockholm, Sweden
Contact:

Post by JH »

I was interested in trying to have a clean black background. Tried some different options. For this BW-picture I used a Nikon plan apo 40x BD 210 mm lens, dark field, and green light (GIF-filter). The green light helped even though this was an APO-lens.


Image

And a 2D stereo made with Zerene.

Image

Best regards
Jörgen Hellberg
Jörgen Hellberg, my webbsite www.hellberg.photo

Online
Saul
Posts: 1644
Joined: Mon Jan 31, 2011 11:59 am
Location: Naperville, IL USA
Contact:

Post by Saul »

Jörgen , result is really beautiful !
Saul

JH
Posts: 1277
Joined: Sat Mar 09, 2013 9:46 am
Location: Vallentuna, Stockholm, Sweden
Contact:

Post by JH »

Thanks Saul :D

Best regards
Jörgen Hellberg
Jörgen Hellberg, my webbsite www.hellberg.photo

WalterD
Posts: 610
Joined: Mon Jul 06, 2015 12:01 pm
Location: Rotterdam, the Netherlands
Contact:

Post by WalterD »

Congratulations, the green light filter turns out to be a very good choice.
:)

Best regards,

Walter

Lou Jost
Posts: 4424
Joined: Fri Sep 04, 2015 7:03 am
Location: Ecuador
Contact:

Post by Lou Jost »

Beautiful monochrome shot. If you want to push this farther, you might want to use violet lasers and a cooled monochrome camera.....you'd achieve 20% more resolution and get smooth backgrounds with no thermal noise.

Post Reply Previous topicNext topic