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Amphipleura pellucida

 
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nanometer



Joined: 30 Apr 2016
Posts: 149
Location: Tucson, AZ

PostPosted: Sat Dec 09, 2017 5:16 pm    Post subject: Amphipleura pellucida Reply with quote

I finally got a decent pic of these today. I used 100x Leitz Floutar with Heine condenser (both oiled) on a Leitz Diavert. I had to put the Heine ring at the transition to darkfield and then add crossed polarizers. Not sure why crossing the polarizers provided the last little nudge though--but I read it works and it did! When polarizers are crossed, they mostly pass blue light, so maybe that's why it works? The camera is the Sony A99 with electronic first curtain shutter on. The camera imager is a very old unit made for a Nikon 35mm camera. The adapter has an M39 thread, so I have a converter to Sony A mount. I burned all the dust out of my lightbox Smile

The slide is made by Klaus Kemp and consists only of Amphipleura pellucida so the coverslip is as close as possible to them. Many thanks to Klaus for making this slide for me!

When I use my scale image, I get about 260nm center-to-center.

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arturoag75



Joined: 05 Feb 2010
Posts: 1585
Location: italy

PostPosted: Sun Dec 10, 2017 2:01 pm    Post subject: Reply with quote

Great result Wink
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KurtM



Joined: 16 Jul 2014
Posts: 62
Location: League City, Texas

PostPosted: Sun Dec 10, 2017 6:35 pm    Post subject: Reply with quote

Day-um. Shocked
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Kurt Maurer
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nanometer



Joined: 30 Apr 2016
Posts: 149
Location: Tucson, AZ

PostPosted: Sat Dec 16, 2017 12:38 pm    Post subject: Reply with quote

Thanks much for looking guys!
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