Today, I am posting my second attempt at stacking photos and giving Photoshop CS6 a second chance at stacking. The subject being photographed this time is a bouquet of orange Gerbera Daisy flowers:

This photo was taken with the Nikkor 16-80mm f/2.8-4E lens mounted on the Nikon D500 camera.
I used the Nikkor 300mm f/4E PF lens mounted on the Nikon D500 camera to take 15 photos of the flowers in small increments from front to back of the bouquet.
As I have posted before, the 300mm lens takes a 1:4 photo at 4 ft. I used my D500 target to determine the close-up capability of this lens at various distances from the front of the lens to the target. Here is a photo of the D500 target I made using Photoshop:

A 1:4 close-up did not include all of the flowers, so I moved the front of the lens back to 6 feet from the flowers. At 6 feet, the lens gives a 1:6 close-up as shown here:

A 1:6 photo with the Nikon D500 camera covers a area approximately 5.5 by 4 inches, enough to see nearly all of the flowers.
Here is the first of the 15 photos showing the bottom left flower petals in focus:

And here is the last of the 15 photos showing the top right flower nearly out of focus:

I used Photoshop CS6 to try and stack the 15 photos. I opened the 15 photos as 15 layers. I selected all 15 layers and aligned them (edit>align layers). Then with all 15 layers aligned, I blended them (edit>blend layers). The blend look very good, so I proceeded to flatten the 15 blended layers into one layer (layer>flatten image). That produced the final stack shown here:

Photoshop successfully stack the 15 photos! This is a stack of 15 photos taken 6 feet from the front of the 300mm lens which is a stack of 1:6 close-up photos.
I wanted to see how sharp or not sharp the stack is, so I cropped the final photo to a 1:3 photo:

Looking good. So I cropped it again to a 1:1.5 photo to get a close look:

This is a really sharp photo that was taken 6 feet from the front of the Nikkor 300mm f/4E PF lens mounted of the Nikon D500 camera. This is really a sharp lens!
This 1:1.5 close-up photo clearly shows the structure of the Orange Gerbera Daisy. The center dark purple pointy rods are the "disk florets". Around the center disk is the "trans florets". These trans florets are purple/yellow banana-like male stamen. Around the trans florets are the yellow flower "ray florets". And finally there are the flowers petals surrounding the inner structure.
So why not take a closer look. I again cropped the final photo to a 1:0.75 close-up which is a 1.33:1 crop (this covers an area approximately 0.7 by 0.5 inches):

For a photo taken 6 feet 8 inches from the Nikon D500 sensor, I would say that is really impressive.
My next post will be using these 15 photos of the Orange Gerbera Daisy to learn stacking using Zerene Stacker. I downloaded the free 30 day trial beta version and used it to stack the Lens Align Ruler. It was successful but the DMAX stack could have been a lot better if I knew how to use the program. So I will spend the day learning Zerene Stacker.
Leon