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Probably Pseudovorticella

 
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carlos.uruguay



Joined: 23 Feb 2012
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Location: Uruguay - Montevideo - America del Sur

PostPosted: Sat Feb 11, 2017 1:00 pm    Post subject: Probably Pseudovorticella Reply with quote

Phase contrast.
Semi-oblique polarized light
Panasonic GH4 Camera
Video link:
https://www.youtube.com/watch?v=i1NtAHWNCh0
Video frames preview:


Regards
carlos


Last edited by carlos.uruguay on Sun Feb 12, 2017 7:12 am; edited 1 time in total
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Bruce Taylor



Joined: 23 Jun 2011
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Location: Wakefield, Quebec / Ottawa, Ontario

PostPosted: Sat Feb 11, 2017 5:01 pm    Post subject: Reply with quote

That's really beautiful, Carlos. Smile

It appears to be Pseudovorticella.
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carlos.uruguay



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PostPosted: Sat Feb 11, 2017 9:18 pm    Post subject: Reply with quote

Thanks Bruce!!!
Can you tell me the difference between vorticella and pseudovorticella
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Bruce Taylor



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PostPosted: Sun Feb 12, 2017 5:50 am    Post subject: Reply with quote

If you closely examine the cell (ideally, with the help of silver stain), the pellicle of Pseudovorticella has a pattern like a wall of bricks, whereas that of Vorticella has simple horizontal striations. This "silverline system" is usually invisible in unstained specimens, but some species of Pseudovorticella also have raised "pellicular tubercles" (beadlike bumps in the surface of the cell) which can be easily seen. These "tubercles" follow the underlying pattern of the silverline system, so if you see them it normally means that your organism is Pseudovorticella. They are quite conspicuous in some species, such as Pseudovorticella monilata.

A simple diagram from Colin R. Curds, British and Other Freshwater Ciliated Protozoa:



I've found that it is often possible to see the silverline system itself without stain, by compressing the cell, opening the diaphragm wide and focusing on the pellicle. Enhancing the contrast in the video software helps, as well.
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carlos.uruguay



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PostPosted: Sun Feb 12, 2017 7:12 am    Post subject: Reply with quote

Thanks Bruce!!
I modified the information on youtube.
Tell me Bruce, only that is the difference between Vorticella and Pseudovorticella? Are differences in its behavior?
I have observed that Pseudovorticella is released from the peduncle without generating posterior cilia
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Bruce Taylor



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PostPosted: Sun Feb 12, 2017 7:54 am    Post subject: Reply with quote

I'm not aware of other morphological or behavioral traits common to the genus. However, in molecular studies Pseudovorticella species group apart from Vorticella (but are entangled with Epicarchesium, which also has a "reticulate" silverline system, i.e. brickwork in the pellicle). Where there are genetic differences, there are bound to be functional differences (in protein synthesis, etc...stuff you can't necessarily see in a microscope!).

As for the zooid spontaneously detaching from the stalk (without forming a telotroch), that is something we also see in Vorticella and many other colonial peritrichs. It is a stress response, caused by environmental disruption, such as being sucked up a pipette, and dropped on a slide under a coverslip!
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carlos.uruguay



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PostPosted: Sun Feb 12, 2017 8:13 am    Post subject: Reply with quote

Thanks!!!!!!!!!!!!!!
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Jacek



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PostPosted: Tue Feb 14, 2017 12:33 pm    Post subject: Reply with quote

Very nice
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carlos.uruguay



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PostPosted: Tue Feb 14, 2017 1:30 pm    Post subject: Reply with quote

Thanks Jacek
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fpelectronica



Joined: 19 Feb 2010
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PostPosted: Wed Feb 15, 2017 7:58 am    Post subject: Reply with quote

Nice and interesting
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carlos.uruguay



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PostPosted: Wed Feb 15, 2017 8:41 am    Post subject: Reply with quote

Thanks Francisco
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Paul92706



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PostPosted: Sat Feb 18, 2017 11:12 pm    Post subject: Reply with quote

very nice!
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carlos.uruguay



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PostPosted: Sun Feb 19, 2017 5:19 am    Post subject: Reply with quote

Thanks Paul
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