Damselfly nymph
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- Charles Krebs
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Damselfly nymph
Here are a couple of shots of a fairly small damselfly nymph.
The first shot is a ventral view showing the multiple-hinged, extendable lower jaw (similar to that seen in dragonfly nymphs). Here it is in the "closed" position but it is thrust out quickly to grasp prey that might otherwise seem out of reach.
The second, lower magnification shot, is a view from above showing the trachea tubes branching through the body.
Nikon MM-11 microscope. Olympus MPlanFL N 10/0.30 objective. Olympus U-SWTR-3. Canon T3i camera.
Nikon MM-11 microscope. Olympus MPlanFL N 5/0.15 objective. Olympus U-SWTR-3. Canon T3i camera.
The first shot is a ventral view showing the multiple-hinged, extendable lower jaw (similar to that seen in dragonfly nymphs). Here it is in the "closed" position but it is thrust out quickly to grasp prey that might otherwise seem out of reach.
The second, lower magnification shot, is a view from above showing the trachea tubes branching through the body.
Nikon MM-11 microscope. Olympus MPlanFL N 10/0.30 objective. Olympus U-SWTR-3. Canon T3i camera.
Nikon MM-11 microscope. Olympus MPlanFL N 5/0.15 objective. Olympus U-SWTR-3. Canon T3i camera.
Last edited by Charles Krebs on Thu Nov 14, 2013 12:52 pm, edited 1 time in total.
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Darkfield
Depending on the size of the disks in my home made darkfield stops, I find I can stop down the condenser iris to some degree before the image blacks out. This reduces the amount of flare in the image.
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA
- Charles Krebs
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Simple darkfield. The microscope and stage I used are like the second picture here:What technique and setup was used ?
http://www.photomacrography.net/forum/v ... hp?t=17656
(But the "tubes" between the microscope and camera body have been replaced with a super-widefield trinocular head).
The condenser is an inexpensive Chinese made stereo darkfield condenser that I mounted on top of an eBay "Right Angle Spy Scope". This was positioned under the stage. A fiber optic light guide is positioned to shine into the angled mirror for the light.
The subject was in a home-made "well" slide, which is nothing more than a plumbing washer glued to a 2x3 inch microscope slide.
I have no magical way of avoiding halos in darkfield stacks, they are a problem for me as well. They are far less of a problem with lower magnifications (such as 5X and 10X here) and also in cases where the subject is rather flat. The legs here are actually not that 3-dimensional and positioned parallel to the camera back so they were not too much of a problem. (Not to say there was not some PS clean-up... there was. )
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darkfield
Dang, Charlie -- that's a crazy setup. Do you have a post for the rht angle gizmo? It looks pretty small.
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA