Playing with fluorescence illumination (images added)

RogelioMoreno · Post by **RogelioMoreno** » Fri Aug 23, 2013 11:36 pm

Here are my first test of my fluorescence illumination system.
All with UV-2B cube and polarized light (composite), stacked, Plan Apo 20x/0.75, Canon T3i mounted on the front port of the TE300.

Micrasterias radiosa by Rogelio Moreno G., on Flickr

Micrasterias rotata by Rogelio Moreno G., on Flickr

Hope you enjoy them.

Rogelio

Jacek · Post by **Jacek** » Fri Aug 23, 2013 11:52 pm

Congratulations, secondary beautiful picture

Litonotus · Post by **Litonotus** » Sat Aug 24, 2013 12:11 am

second one is great (: fluorescence becomes popular here (:

Hindpool · Post by **Hindpool** » Sat Aug 24, 2013 11:47 pm

The detail is superb and especially so in the second photo. Wonderful work Rogelio - and to read that you are just starting with Fluorescence makes me wonder just how much more is to come! Congratulations.

sebba28 · Post by **sebba28** » Sun Aug 25, 2013 5:37 am

Exellent shots, Rogelio
I can"t wait to see more your fluorescence illumination pictures.

discomorphella · Post by **discomorphella** » Sun Aug 25, 2013 10:13 am

Hi Rogelio,

Superb results. I hope you enjoy epifluorescence as much as I do. Next we'll get you and Waldo using fluorescent staining as well....
What were the individual exposure times?

David

g4lab · Post by **g4lab** » Sun Aug 25, 2013 11:53 am

shameless but non commercial sales promotion
Anybody needing a fluorescent light source should send me a private message. I have HBO arc lamp outfits available and also the Lumen Dynamics X-Cite metal halide which Rogelio is using. end promotion
Beautiful job , as always, Rogelio!

RogelioMoreno · Post by **RogelioMoreno** » Mon Aug 26, 2013 6:21 am

Thank you very much for your kind words.

David, I will enjoy it. Are the fluorescent staining safe?
The fluorescence were 3.2 seconds with the X-Cite's diaphragm set to 12% (to avoid photo-bleach, I have to try with greater aperture), the polarized with flash 1/200.

Gene, thank you for the deal of the X-Cite illuminator.

Rogelio

René · Post by **René** » Mon Aug 26, 2013 6:35 am

Wow, these are fantastic! Thansk for showing!

René

luchalagos · Post by **luchalagos** » Mon Aug 26, 2013 8:35 am

beautiful Rogelio!! congrats!!!

pwnell · Post by **pwnell** » Mon Aug 26, 2013 11:37 am

Hey Rogelio,

These are really great. Seems like you managed very well to prevent the fluorescence from the chloroplast leaking - these are sharp.

Ecki · Post by **Ecki** » Mon Aug 26, 2013 12:52 pm

Very nice!

RogelioMoreno · Post by **RogelioMoreno** » Mon Aug 26, 2013 2:59 pm

Thank you again for your comments.

Anybody interested in knowing how to overlay fluorescence and brightfield (also can be polarized, DIC, phase contrast, etc) check the following tutorial:

http://www.youtube.com/watch?v=WWQhMnC3BKU

Rogelio

RogelioMoreno · Post by **RogelioMoreno** » Tue Aug 27, 2013 3:53 pm

Here a few more.

Closterium by Rogelio Moreno G., on Flickr

Micrasterias furcata by Rogelio Moreno G., on Flickr

Spirogyra by Rogelio Moreno G., on Flickr

Rogelio

Litonotus · Post by **Litonotus** » Tue Aug 27, 2013 9:20 pm

abslolutely brilliant and visually stunning! (: