Plants & Algae

[pwnell]

20130810-DSLR_IMG_1017-Edit.jpg by pwnell, on Flickr
Leaf, 2x, EPI-XC-POL, HF B


20130810-DSLR_IMG_1113-Edit.jpg by pwnell, on Flickr
Pine needles, 2x, FLUO-C4, cellSens deconvolve spherical


20130810-DSLR_IMG_1116-Edit.jpg by pwnell, on Flickr
Pine needles, 40x/0.6, EPI-XC-POL, HF C


20130812-DSLR_IMG_0024.jpg by pwnell, on Flickr
Algae from minnekhada park, 20x, FLUO-C4


20130812-DSLR_IMG_0030.jpg by pwnell, on Flickr
Algae from minnekhada park, 40x, DIC


20130812-DSLR_IMG_0032.jpg by pwnell, on Flickr
Algae from minnekhada park, 40x, DIC


20130812-DSLR_IMG_0035.jpg by pwnell, on Flickr
Algae from minnekhada park, 40x, FLUO-C4


20130812-DSLR_IMG_0037.jpg by pwnell, on Flickr
Algae from minnekhada park, 40x, POL


20130812-DSLR_IMG_0081-Edit.jpg by pwnell, on Flickr
Algae from minnekhada park, 40x,DIC, HF B (8,8)

[emsiem]

Beautiful!

[RogelioMoreno]

Beautiful set!

I would like to see the second one without deconvolution (to compare).

Rogelio

[pwnell]

Thanks. Here (the difference is more striking at 100%):

[Jacek]

Beautiful as always

[discomorphella]

Excellent autofluorescence shots. One thing I really like about having an aberration model in the deconvolution to take out the spherical terms is that you don't have to really fuss with the correction collar...of course you have to take a large stack of images, nature doesn't give anything away for free...next you'll have to try mixing in some Calcofluor to stain the cell walls into your pond slide (and then, WITH PROPER GLOVES and safety goggles etc, some TOTO or DAPI to light up the nuclei).

David

[pwnell]

Thanks. My problem is that I cannot get any of those stains shipped to me as they will only be sold to educational institutions.

[jswatts]

Yep, excellent images. FYI, calcofluor is a paper whitener, so you might be able to find another source for it.

[Cactusdave]

Ebay item 350101958297 is calcofluor white. The seller also has other fluorescent stains available.

[carlos.uruguay]

Very beautiful