Fern stomata

pwnell · Post by **pwnell** » Sun Aug 11, 2013 11:04 am

There was a discussion surrounding the black "bars" in the fluorescence shot of these fern stomata before. This may be a better photo showcasing the interesting pattern.

20130810-DSLR_IMG_0837.jpg by pwnell, on Flickr
Fern stomata, 40x/0.6,EPI-XC-POL, HF B (15,3)

20130810-DSLR_IMG_0866.jpg by pwnell, on Flickr
Fern stomata, 40x/0.6,FLUO-C4, HF B (40,2)

RogelioMoreno · Post by **RogelioMoreno** » Sun Aug 11, 2013 12:06 pm

Nice, specially the last one.

Rogelio

Cyclops · Post by **Cyclops** » Sun Aug 11, 2013 2:46 pm

Very interesting!

Pau · Post by **Pau** » Mon Aug 12, 2013 12:40 am

Nice images as usual, Waldo. I like the comparison with the same field.

Jacek · Post by **Jacek** » Mon Aug 12, 2013 3:51 am

beautiful

Fredlab · Post by **Fredlab** » Mon Aug 12, 2013 7:06 am

Excellent shot
As always.

Fluo is amazing.
You must try with only epidermis (chlorophyl in stomatic cell only)

pwnell · Post by **pwnell** » Mon Aug 12, 2013 9:40 am

Thanks. What do you mean by chlorophyll in stomatic cell only? Do you mean a more magnified photo?

Fredlab · Post by **Fredlab** » Mon Aug 12, 2013 10:10 am

Epidermic cells are not chlorophyllian, only stomatic cells contain chloroplasts.
I think, epidermis (easy to collect) with stomates, in fluorescence, will be nice.

pwnell · Post by **pwnell** » Mon Aug 12, 2013 10:24 am

Where would I collect these kind of subjects? I thought chloroplasts fill the epidermis of all plant leaves?

Cyclops · Post by **Cyclops** » Mon Aug 12, 2013 11:06 am

pwnell wrote: I thought chloroplasts fill the epidermis of all plant leaves?

So did I, considering chlorophyll is what makes plants green! Plus in most plants the stomata are only found on the underside to reduce transpiration in hot weather. If only stomatic cells had chlorophyll then most plants would appear very pale!

Fredlab · Post by **Fredlab** » Mon Aug 12, 2013 11:12 am

pwnell wrote:Where would I collect these kind of subjects? I thought chloroplasts fill the epidermis of all plant leaves?

Simply tear sheet obliquely. A white "skin" will appear at the torn area ... with fine forceps, you removed a fragment that can be even greater (bottom panel - it comes off easily)

(panoramic shot - bigger size - HERE)