Quiz...What is it? (We have a correct answer!)
Moderators: rjlittlefield, ChrisR, Chris S., Pau
- Charles Krebs
- Posts: 5865
- Joined: Tue Aug 01, 2006 8:02 pm
- Location: Issaquah, WA USA
- Contact:
Quiz...What is it? (We have a correct answer!)
Quite an easy one I think. Live subject. Scale bar might help.
Nikon MM-11 microscope, Olympus LPLFLN 50/0.50 objective. Canon T3i. 50X on sensor.
Nikon MM-11 microscope, Olympus LPLFLN 50/0.50 objective. Canon T3i. 50X on sensor.
Last edited by Charles Krebs on Sun Jun 09, 2013 12:04 am, edited 1 time in total.
- rjlittlefield
- Site Admin
- Posts: 23625
- Joined: Tue Aug 01, 2006 8:34 am
- Location: Richland, Washington State, USA
- Contact:
- Charles Krebs
- Posts: 5865
- Joined: Tue Aug 01, 2006 8:02 pm
- Location: Issaquah, WA USA
- Contact:
Not a rotifer. There was a reason why I needed to use a "harder" directional light on this rather than my normal diffusers. (That may be an extremely vague hint).
I've not seen this view posted before (... actually I've never seen an image of this detail before... but we all know the subject well).but I'm wondering if the very directional lighting is making this subject look different from other shots we've seen.
- rjlittlefield
- Site Admin
- Posts: 23625
- Joined: Tue Aug 01, 2006 8:34 am
- Location: Richland, Washington State, USA
- Contact:
Hhmm....
Directional light illuminating just the interior of a 0.093 mm diameter tube with inward-pointing teeth on the inside surface, belonging to a live subject.
I'm thinking this subject was light-sensitive and the illumination was partly to keep it quiet.
But what sort of tube would have a structure like that?
Mosquito larva siphon, or pupa trumpet?
--Rik
Directional light illuminating just the interior of a 0.093 mm diameter tube with inward-pointing teeth on the inside surface, belonging to a live subject.
I'm thinking this subject was light-sensitive and the illumination was partly to keep it quiet.
But what sort of tube would have a structure like that?
Mosquito larva siphon, or pupa trumpet?
--Rik
- Charles Krebs
- Posts: 5865
- Joined: Tue Aug 01, 2006 8:02 pm
- Location: Issaquah, WA USA
- Contact:
Mosquito pupa trumpet!
Here's a top view with a 10X...
Another 10X from the side. It was too active for me to get a complete front to back stack, so I concentrated on the eye area...
Here's an old shot from about 3 years ago that gives a better perspective to the location of these respiratory tubes...
The reason I couldn't diffuse the light more was that in order to keep the pupa somewhat centered under the microscope objective it was in a small petri dish in a small "dome" of water. Because of the water surface shape I couldn't use any diffusers/reflectors without some serious reflection problems. When the pupa was at rest it would rise to the top of the water curvature and give me enough time for a small image stack. It did drift around a tiny bit between shots (looked like more than "a bit" with the 50X ), but Zerene did a great job of aligning things.
Here's a top view with a 10X...
Another 10X from the side. It was too active for me to get a complete front to back stack, so I concentrated on the eye area...
Here's an old shot from about 3 years ago that gives a better perspective to the location of these respiratory tubes...
The reason I couldn't diffuse the light more was that in order to keep the pupa somewhat centered under the microscope objective it was in a small petri dish in a small "dome" of water. Because of the water surface shape I couldn't use any diffusers/reflectors without some serious reflection problems. When the pupa was at rest it would rise to the top of the water curvature and give me enough time for a small image stack. It did drift around a tiny bit between shots (looked like more than "a bit" with the 50X ), but Zerene did a great job of aligning things.
- arturoag75
- Posts: 1600
- Joined: Fri Feb 05, 2010 1:05 am
- Location: italy
- Contact:
- rjlittlefield
- Site Admin
- Posts: 23625
- Joined: Tue Aug 01, 2006 8:34 am
- Location: Richland, Washington State, USA
- Contact:
-
- Posts: 2979
- Joined: Fri Nov 20, 2009 11:24 am
- Location: Panama
- myriophyllum
- Posts: 151
- Joined: Wed Sep 05, 2007 5:47 pm
- Location: Schaumburg, north of Germany
- Contact:
it's just amazing how creative you are (: world top class (:
my FB page
I'm looking for the the extemely rare V-IM magnification changer for the E800 scope. If you have seen a listing or have one for sale please let me know.
I'm looking for the the extemely rare V-IM magnification changer for the E800 scope. If you have seen a listing or have one for sale please let me know.
- rjlittlefield
- Site Admin
- Posts: 23625
- Joined: Tue Aug 01, 2006 8:34 am
- Location: Richland, Washington State, USA
- Contact:
It's a tribute to my limited imagination and Charlie's excellent clues. After Floscularia, aquatic insects were the only things I could think of that might have structures like this and would be described as "live subject". Of those, mosquito larvae and pupae were the only ones that fit the clue "we all know the subject well". Web search on other images didn't let me rule out either one, so I went for both.curt0909 wrote:Rik, I don't know how you got that on your second guess.
In fairness, "pupa trumpet" was my third guess. I posted just "larva siphon?" first, then a couple of minutes later edited the post to include pupa trumpet.
--Rik