www.photomacrography.net :: View topic - Dividing Arcella, Frontonia, Paramecium pellicle, Holospora
www.photomacrography.net Forum Index
An online community dedicated to the practices of photomacrography, close-up and macro photography, and photomicrography.
Photomacrography Front Page Amateurmicrography Front Page
Old Forums/Galleries
 
 FAQFAQ   SearchSearch   MemberlistMemberlist   UsergroupsUsergroups   RegisterRegister 
 ProfileProfile   Log in to check your private messagesLog in to check your private messages   Log inLog in 
Dividing Arcella, Frontonia, Paramecium pellicle, Holospora

 
Post new topic   Reply to topic    www.photomacrography.net Forum Index -> Photography Through the Microscope
View previous topic :: View next topic  
Author Message
Psi Wavefunction



Joined: 04 Dec 2009
Posts: 12
Location: Halifax

PostPosted: Tue May 07, 2013 9:15 am    Post subject: Dividing Arcella, Frontonia, Paramecium pellicle, Holospora Reply with quote

Back with a couple goodies!
And news: off to Halifax to start a MSc on protist diversity and cell structure in June; among my first tasks will be to bring the scope up to sexy standards. And then learn some TEM! (are electron micrographs allowed here, by the way?)

A dividing arcellinid (Arcella sp. here): the new shell is the clear one; thecae tend to "rust" over time and turn brown.


Frontonia full of tasty diatoms!


The giant Bursaria are notable for rapidly deciliating ciliates, like Paramecia, upon contact -- and completely by the time the ciliates pass the gullet. Bursarias also have this annoying habit of exploding when you look at them, thus releasing half-digested and not-yet-digested Paramecia into the surroundings. Conveniently enough, those Paramecia are free of cilia, so you can get a really nice crisp view of the pellicle!


Paramecium with a sporulating Holospora infection, from culture. The large refractile structure is... no, not the macronucleus. It is a micronucleus severely bloated by bacterial spores. Holospora spend part of their lives in nuclei, feeding on the chromatin and later sporulating. It's just one example of bacterial endosymbionts flourishing inside protist cells; not all are parasitic either.


Incidentally, I found a clump of Peritrichs with bacteria in the macronucleus (some dividing; arrowhead); this has apparently not been recorded in the literature before, and I lost the critters before getting around to culture them =(


Ok, this is probably enough to slow down the loading time. More later!
Cheers,
-Psi-
_________________
http://skepticwonder.fieldofscience.com
http://blogs.scientificamerican.com/ocelloid
Back to top
View user's profile Send private message Visit poster's website
pwnell



Joined: 18 Dec 2009
Posts: 2001
Location: Tsawwassen, Canada

PostPosted: Tue May 07, 2013 10:03 am    Post subject: Reply with quote

Very interesting, thanks.
Back to top
View user's profile Send private message
Ecki



Joined: 13 Aug 2008
Posts: 775
Location: Cape Town, South Africa

PostPosted: Tue May 07, 2013 11:48 am    Post subject: Reply with quote

Hi Psi,

Great to read you here! I enjoyed your blog a lot. SEM/TEM images are always welcome (I showed one today). In fact, I will be posting a lot of SEM images myself in a couple of months.

The Holospora infection is very interesting. In one of my amoeba cultures I found all amoeba with a strange "Nebenk├Ârper" in the nucleus.

http://www.photomacrography.net/forum/viewtopic.php?t=13471

Do you have any idea what this could be?

Regards from Berlin,
Ecki
_________________
www.penard.de
www.wunderkanone.de
www.flickr.com/wunderkanone
Back to top
View user's profile Send private message Visit poster's website
rjlittlefield
Site Admin


Joined: 01 Aug 2006
Posts: 20350
Location: Richland, Washington State, USA

PostPosted: Tue May 07, 2013 1:30 pm    Post subject: Re: Dividing Arcella, Frontonia, Paramecium pellicle, Holosp Reply with quote

Psi Wavefunction wrote:
And then learn some TEM! (are electron micrographs allowed here, by the way?)

Just to confirm -- yes, absolutely. I have a particular interest in comparisons of optical & electron micrographs, to help us understand what can and cannot be seen with optical. Most readers will never have a chance to work directly with electron scopes, so it's nice to know what we're missing. Very Happy

--Rik
Back to top
View user's profile Send private message Visit poster's website
Bruce Taylor



Joined: 23 Jun 2011
Posts: 792
Location: Wakefield, Quebec / Ottawa, Ontario

PostPosted: Tue May 07, 2013 5:18 pm    Post subject: Reply with quote

Ecki wrote:
I enjoyed your blog a lot.


Since you're using the past tense, you must be referring to Skeptic Wonder (not dead, apparently...merely encysted Smile ). In case you've missed it, here is her "new" blog: http://blogs.scientificamerican.com/ocelloid/
Back to top
View user's profile Send private message Visit poster's website
RogelioMoreno



Joined: 20 Nov 2009
Posts: 2962
Location: Panama

PostPosted: Fri May 10, 2013 3:56 pm    Post subject: Reply with quote

Very interesting!

I could id the Apocarchesium thanks to your blog. Very Happy

Rogelio
Back to top
View user's profile Send private message Send e-mail
carlos.uruguay



Joined: 23 Feb 2012
Posts: 5284
Location: Uruguay - Montevideo - America del Sur

PostPosted: Sat May 11, 2013 7:08 am    Post subject: Reply with quote

Very interesting! Thank you for sharing!!!
Back to top
View user's profile Send private message Send e-mail Visit poster's website
Display posts from previous:   
Post new topic   Reply to topic    www.photomacrography.net Forum Index -> Photography Through the Microscope All times are GMT - 7 Hours
Page 1 of 1

 
Jump to:  
You cannot post new topics in this forum
You cannot reply to topics in this forum
You cannot edit your posts in this forum
You cannot delete your posts in this forum
You cannot vote in polls in this forum


Powered by phpBB © 2001, 2005 phpBB Group