Unexpected colors
Moderators: rjlittlefield, ChrisR, Chris S., Pau
- rjlittlefield
- Site Admin
- Posts: 23605
- Joined: Tue Aug 01, 2006 8:34 am
- Location: Richland, Washington State, USA
- Contact:
Unexpected colors
Following the theme of "unexpected colors", and since we haven't a puzzle piece for a while, I offer the following:
In the spirit of disclosing significant changes in postprocessing, I confess that I applied a curves adjustment to increase contrast.
Here is the adjustment:
Pressing the Auto button for curves adjustment removed a lot of the subject's natural background color:
Here is the adjustment producing that image:
And finally, here is the original unadjusted image:
Optical setup was 10X Mitutoyo objective with Thorlabs ITL200 tube lens, to a Canon T1i sensor. The objective was significantly stopped down and the stack was shot at 20 microns step size, only 8 frames, run through Zerene Stacker using DMap. Illumination was halogen, using a single bundle of optical fiber with Kleenex tissue wrapped around its head, the light source positioned about midway along the objective, just far enough off axis to keep the objective from shadowing the subject.
Now the obvious question: what is this beautiful object??
--Rik
In the spirit of disclosing significant changes in postprocessing, I confess that I applied a curves adjustment to increase contrast.
Here is the adjustment:
Pressing the Auto button for curves adjustment removed a lot of the subject's natural background color:
Here is the adjustment producing that image:
And finally, here is the original unadjusted image:
Optical setup was 10X Mitutoyo objective with Thorlabs ITL200 tube lens, to a Canon T1i sensor. The objective was significantly stopped down and the stack was shot at 20 microns step size, only 8 frames, run through Zerene Stacker using DMap. Illumination was halogen, using a single bundle of optical fiber with Kleenex tissue wrapped around its head, the light source positioned about midway along the objective, just far enough off axis to keep the objective from shadowing the subject.
Now the obvious question: what is this beautiful object??
--Rik
- Craig Gerard
- Posts: 2877
- Joined: Sat May 01, 2010 1:51 am
- Location: Australia
- rjlittlefield
- Site Admin
- Posts: 23605
- Joined: Tue Aug 01, 2006 8:34 am
- Location: Richland, Washington State, USA
- Contact:
Yes. The subject is not completely smooth, so I added a coverslip with glycerin to fill the gap and avoid direct reflections of the fiber bundle from surface texture.Craig Gerard wrote:Are you shooting through some type of immersion oil?
The Mitutoyo may not the best objective to use for this situation, but I did not realize the problem until I was already set up to shoot with that lens. On the other hand, the Mitutoyo allows illumination closer to the axis than any of my other 10X lenses, which has some importance in bringing out the full intensity of the colors. I shot it only with this one objective, so I'm not sure about all the tradeoffs.
--Rik
- rjlittlefield
- Site Admin
- Posts: 23605
- Joined: Tue Aug 01, 2006 8:34 am
- Location: Richland, Washington State, USA
- Contact:
- rjlittlefield
- Site Admin
- Posts: 23605
- Joined: Tue Aug 01, 2006 8:34 am
- Location: Richland, Washington State, USA
- Contact:
- rjlittlefield
- Site Admin
- Posts: 23605
- Joined: Tue Aug 01, 2006 8:34 am
- Location: Richland, Washington State, USA
- Contact:
- rjlittlefield
- Site Admin
- Posts: 23605
- Joined: Tue Aug 01, 2006 8:34 am
- Location: Richland, Washington State, USA
- Contact:
-
- Posts: 5090
- Joined: Sun Jan 15, 2012 12:31 pm
- rjlittlefield
- Site Admin
- Posts: 23605
- Joined: Tue Aug 01, 2006 8:34 am
- Location: Richland, Washington State, USA
- Contact:
Nope, neither of those, but you're giving me great ideas of other things to look at.
The material shown retains much more of its natural structure than chiccarones (fried pork skin). I think that's true for sausage casings too, but I'm not so familiar with how those are processed.
I understand from my readings that the same colors can be found in related products that are completely fresh. I haven't confirmed that personally.
More hints... This stuff is tasty and nutritious, but it's banned by certain religions and ethical standards. I spotted the colors while getting ready to eat and saved out this little piece to photograph. The material does not usually appear as shown here. When the colors are noticed, they often prompt questions about whether the sample is still good to eat.
--Rik
The material shown retains much more of its natural structure than chiccarones (fried pork skin). I think that's true for sausage casings too, but I'm not so familiar with how those are processed.
I understand from my readings that the same colors can be found in related products that are completely fresh. I haven't confirmed that personally.
More hints... This stuff is tasty and nutritious, but it's banned by certain religions and ethical standards. I spotted the colors while getting ready to eat and saved out this little piece to photograph. The material does not usually appear as shown here. When the colors are noticed, they often prompt questions about whether the sample is still good to eat.
--Rik